- Open Access
JARID2 and the PRC2 complex regulate skeletal muscle differentiation through regulation of canonical Wnt signaling
Epigenetics & Chromatin volume 11, Article number: 46 (2018)
JARID2 is a non-catalytic member of the polycomb repressive complex 2 (PRC2), which is known to regulate developmental target genes in embryonic stem cells. Here, we provide mechanistic insight into the modulation of Wnt signaling by JARID2 during murine skeletal muscle differentiation.
We show that JARID2 is expressed in proliferating myoblasts, but downregulated upon muscle differentiation. Unexpectedly, depletion of JARID2 or the catalytic subunit of the PRC2 complex, EZH2, inhibited differentiation, suggesting that JARID2 and the PRC2 complex are required to initiate this process. Expression of the myogenic regulatory factors required to promote differentiation, MYOD and MYOG, was downregulated in the absence of JARID2, even though decreases in the methylation of histone H3 lysine 27 (H3K27me3) were observed on both promoters. We found that activation of the Wnt signaling pathway upregulated MYOD and restored differentiation. Activation of the Wnt pathway in JARID2 depleted cells caused β-catenin to translocate to the nucleus, where it bound to and activated the Myod1 promoter. We show that the Wnt antagonist SFRP1 is highly upregulated in the absence of JARID2 and is a direct target of JARID2 and the PRC2 complex. Ectopic expression of SFRP1 blocked MYOD and late muscle gene expression and inhibited the translocation of β-catenin to the nucleus. Finally, we show that JARID2 and SFRP1 are inversely correlated in melanoma, confirming that the JARID2-mediated repression of SFRP1 extends beyond skeletal muscle and has important implications in many cellular systems, including cancer.
We show that JARID2 and the PRC2 complex regulate muscle differentiation by modulating Wnt signaling through the direct repression of Wnt antagonists.
Skeletal muscle differentiation is controlled by four highly related basic helix-loop-helix proteins known as the myogenic regulatory factors (MRFs), which include MYF5, MYOD, MYOG and MYF6. The process of differentiation is a tightly regulated process that is modulated by many signaling pathways, including the Wnt signaling pathway. Wnt functions through both a canonical and non-canonical pathway, and both contribute to skeletal muscle growth and homeostasis . In the canonical pathway, binding of Wnt proteins to the frizzled (FZD) receptor activates β-catenin, which is normally associated with its own degradation complex that includes the serine–threonine kinase glycogen synthase kinase 3 (GSK3), AXIN and adenomatous polyposis coli (APC). Phosphorylation of β-catenin by GSK3 within the destruction complex leads to its ubiquitin-mediated proteasomal degradation. However, binding of Wnt ligand to FZD receptors activates disheveled (DSH) and heterotrimeric G proteins that lead to recruitment of AXIN to the intracellular domain of the FZD co-receptor, low-density lipoprotein receptor-related protein (LRP). This leads to disassembly of the destruction complex releasing free β-catenin in the cytoplasm, which later translocates to the nucleus and activates gene expression in concert with the T cell factor (TCF) and lymphoid enhancer-binding factor (LEF) families of transcription factors. One of the targets of β-catenin has been shown to be Myod1 .
Polycomb repressor proteins have important developmental roles and play essential roles in skeletal muscle specification. The two major polycomb complexes, polycomb repressive complex 1 (PRC1) and polycomb repressive complex 2 (PRC2), are responsible for the ubiquitination of histone H2A on lysine 119 (H2AK119) and the methylation of H3 lysine 27 (H3K27), respectively [3,4,5]. Methylation of H3K27 recruits PRC1, which then catalyzes the ubiquitination of H2AK119. The methylation of H3K27 by PRC2 requires the presence of one of the enhancer of Zeste proteins, EZH1 or EZH2. Di- and trimethylation of H3K27 is primarily associated with facultative heterochromatin, while mono-methylation is associated with intergenic heterochromatin. Promoters and enhancers of many lineage-specific genes contain di- and trimethylated H3K27, and many of these elements lose the methylation profile upon differentiation (reviewed in ). In skeletal muscle, EZH2 is co-expressed with PAX7, which is essential for satellite cell specification and maintenance  and appears to be required for homeostasis of the satellite cell pool . Consistent with this role, EZH2 expression is downregulated with the differentiation of myoblasts and the activation of muscle gene expression .
JARID2 is the catalytically inactive founding member of the jumonji family of proteins. JARID2 is a sub-stoichiometric component of PRC2 that appears to optimize the enzymatic activity of PRC2 and is thought to function in recruiting PRC2 . JARID2 is known to be involved in targeting the PRC2 complex, but how JARID2 recognizes target sites is unclear. We have shown that JARID2 is recruited by interferon-gamma-stimulated CIITA to repress muscle-specific genes . In embryonic stem (ES) cells, deletion of Jarid2 shows a severe differentiation block due to an upregulation of Nanog mRNA and an inhibition of Wnt signaling . ChIP sequencing data for JARID2 in ES cells showed JARID2 bound to 1337 promoters, including genes critical for Wnt signaling and Wnt-related pathways . Included in this group were two Wnt antagonists, secreted frizzled related protein 1 (Sfrp1) and naked cuticle homolog 1 (Nkd1). Sfrp1 is one of five highly related proteins in mouse and human that encode secreted Wnt antagonists [14,15,16], and Nkd1 encodes a disheveled binding protein that acts as a negative regulator of Wnt . Sfrp1 was one of a panel of Wnt signaling genes shown to be upregulated in embryonic stem (ES) cells upon JARID2 deletion .
To understand the role of JARID2 in skeletal muscle more clearly, we sought to understand the expression and function of JARID2 during muscle differentiation in this work. We show that, as previously shown for EZH2, JARID2 is downregulated upon skeletal muscle differentiation. We anticipated that depletion of JARID2 and EZH2 would promote differentiation, but unexpectedly, we found that JARID2 and EZH2 were instead required to initiate differentiation. Depletion of Jarid2 mRNA blocked the expression of MYOD and MYOG. We found that restoration of Wnt signaling restored MYOD expression and muscle differentiation, and we show that JARID2 and the PRC2 complex directly repress an antagonist of the Wnt signaling pathway, Sfrp1. Thus, we show the essential role that JARID2 and the PRC2 complex play in the differentiation of skeletal muscle and establish that they act to modulate Wnt signaling in muscle. Finally, we show that JARID2 and SFRP1 are negatively correlated in cancer and correlate with overall survival and life span, suggesting that the repression of SFRP1 by JARID2 has important implications outside of skeletal muscle as well.
To establish the function of JARID2 in skeletal muscle, we first sought to understand the expression of JARID2 in a time course of muscle differentiation using C2C12 cells, an immortalized murine cell line model of myogenesis. We found that Jarid2 mRNA was expressed in proliferating myoblasts, but was downregulated upon muscle differentiation (Fig. 1a). Similar results were observed for the protein expression of JARID2 (Fig. 1b). The expression of myosin heavy chain (MHC) was used as a marker of differentiation. To confirm that these results reflected events occurring in normal skeletal muscle, we repeated the experiment using freshly derived primary myoblasts. We found that JARID2 was downregulated upon differentiation in these cells as well (Fig. 1c). To determine whether JARID2 influenced myogenesis, we depleted Jarid2 mRNA with a series of shRNA constructs in C2C12 cells. The depletion of Jarid2 mRNA by each shRNA construct was confirmed at the level of RNA (Fig. 1d) and protein (Fig. 1e). We selected the shRNA constructs shJarid2-1 and shJarid2-3 for further analysis. The results from both lines were similar, so only the results from shJarid2–3 are further reported here. When the cell lines were subjected to differentiation conditions, we found that differentiation was strongly inhibited in the JARID2 depleted cell line as confirmed by immunohistochemistry for MHC (Fig. 1f). Additionally, RNA analysis for the expression of a three differentiation-specific markers; myosin light chain phosphorylatable, fast (Mylpf), troponin I, skeletal, fast 2 (Tnni2) and leiomodin 2 (Lmod2) confirmed the expression block to differentiation-specific genes (Fig. 1g).
JARID2 is a non-catalytic member of the PRC2 complex, so we next asked whether this effect was a result of the loss of JARID2, or a loss of PRC2 complex activity. We did this by depleting the catalytic subunit of PRC2, EZH2, using shRNA constructs. We identified a shRNA, shEzh2-1, that led to a significant depletion of Ezh2 mRNA that could be observed at the level of RNA (Fig. 2a) and protein (Fig. 2b). When subjected to differentiation conditions, the EZH2 depleted cell line also showed an inhibition of differentiation as assayed by MHC immunohistochemistry (Fig. 2c). RNA analysis also showed a significant reduction in the expression of Mylpf, Tnni2 and Lmod2 (Fig. 2d). The block to differentiation is not as robust as observed for JARID2 depleted lines, but JARID2 was also depleted more efficiently than was EZH2, complicating any comparison of the effects in the two cell lines. However, the data clearly show that both JARID2 and EZH2 are required to initiate differentiation.
This requirement was very surprising, as we had anticipated that the loss of the PRC2 complex would enhance differentiation. Many differentiation-specific genes are controlled by the chromatin target of PRC2, methylation of histone H3 lysine 27 (H3K27me) . Myogenin (MYOG), the MRF required for myoblast fusion and differentiation, is known to be a target of the PRC2 complex . Thus, we examined the expression of Myog in the JARID2 depleted line and found that MYOG was not upregulated upon differentiation in the JARID2 depleted line. This result could be observed at the level of both RNA (Fig. 3a) and protein (Fig. 3b). Next, we examined the expression of Myod1 and found that MYOD was also strongly repressed in the JARID2 depleted line at the level of both RNA (Fig. 3c) and protein (Fig. 3d). JARID2 has been shown to be both inhibitory for PRC2 complex activity and required for PRC2 targeting, suggesting that JARID2 acts to “fine-tune” PRC2 complex activity [13, 19]. To determine how depletion of JARID2 impacted the activity of the PRC2 complex on the promoters of Myod1 and Myog, we performed chromatin immunoprecipitation (ChIP) assays with antibodies against H3K27me3 and examined the enrichment on the Myod1 and Myog promoters in the presence or depletion of JARID2. MyoD1 is regulated by several major upstream cis-elements including the distal regulatory region (DRR), core enhancer (CE) and proximal regulatory region (PRR) [20, 21]. We found that H3K27 methylation could be observed at the DRR, CE and PRR of the Myod1 promoter and that depletion of JARID2 leads to a loss of H3K27me3 on each of these regions (Additional file 1: Figure S1 a–c). H3K27 methylation was also observed on the myogenin promoter in a previously identified region 1.5-kb upstream of the transcriptional start site , and depletion of JARID2 also led to a reduction of H3K27me3 in this region (Additional file 1: Figure S1 d). No change in methylation was observed on the promoter proximal region of the myogenin promoter (Additional file 1: Figure S1 e). As a control for these experiments, we show that H3K27me3 is observed on the HoxB7 promoter (Additional file 1: Figure S1 f), a known target of the PRC2 complex . We show here that methylation of the HoxB7 promoter is independent of JARID2. Significantly, we also show here that JARID2 is required to guide the PRC2-mediated H3K27 methylation responsible for repressing both Myod1 and Myog and that JARID2 does not inhibit PRC2 complex activity on these promoters. In cardiac cells, JARID2 has been shown to guide histone H3 lysine 9 (H3K9me) methylation to target genes . Thus, we also examined the enrichment of H3K9me on the Myod1 and Myog promoters in the presence or depletion of JARID2. We found no decrease in H3K9me on the Myod1 promoter (Additional file 1: Figure S1 g–i) or the promoter proximal region of the Myog promoter (Additional file 1: Figure S1 j). A reduction in H3K9me was observed on the 1.5-kb upstream region of the myogenin promoter (Additional file 1: Figure S1 k), indicating that JARID2 may play a role in directing H3K9me in this region. However, our data show that the loss of the repressive methylation of H3K27 or H3K9 is not sufficient for activation of the MRFs. Instead, our data suggested that JARID2 and the PRC2 complex must have an indirect role in activating Myod1 and Myog upon differentiation.
These results were very surprising given the previously published work, showing that transient siRNA depletion of another component of the PRC2 complex, SUZ12, led to a precocious activation of myogenin RNA . Other work has shown no change in Myog mRNA expression upon a transient siEZH2 depletions and a delay in Myog mRNA expression upon transient siSUZ12 depletion . To reconcile our results to these studies, we performed transient transfections with the shJarid2 and shEzh2 constructs used to construct our stable cell lines and examined gene expression profiles 24 or 48 h post-transfection. As anticipated, Jarid2 mRNA (Fig. 3e) and Ezh2 mRNA (Fig. 3f) were depleted by the respective shRNA constructs. We found that Myog mRNA was upregulated 24 h post-transfection, but Myog mRNA levels were returned to normal levels by 48 h (Fig. 3h). Myod1 mRNA was modestly downregulated after 24 h, but restored to normal or slightly elevated levels by 48 h (Fig. 3g). These results strongly suggest that the sustained depletion of Jarid2 mRNA and Ezh2 mRNA in our stable cell lines is required for the inhibition of MYOD and MYOG we observe.
To determine whether the loss of MYOD was responsible for the block to differentiation we observed, we asked whether ectopic expression of MYOD in JARID2 depleted cells could rescue differentiation. An expression construct for Myod1 (cDNA) was transiently expressed in JARID2 depleted cells, and we found that, as anticipated, Myod1 mRNA expression was observed (Additional file 2: Figure S2 a). We next examined Myog mRNA expression and found that expression of MYOD also upregulated Myog mRNA (Additional file 2: Figure S2 b). Analysis of a differentiation-specific marker, Mylpf, also showed upregulation upon expression of MYOD (Additional file 2: Figure S2 c). These data strongly suggested that the inhibition of Myod1 was largely responsible for the block to differentiation in JARID2 depleted cells.
The regulation of Myod1 is complex and impacted by many signaling pathways, including the Wnt signaling pathway (reviewed in ). To determine whether modulation of Wnt signaling could impact the JARID2-mediated block to differentiation, we treated JARID2 depleted cells with a chemical inhibitor of GSK3β, lithium chloride (LiCl). Inhibition of GSK3β by LiCl is known to enhance myotube formation in C2C12 cells . Treatment with NaCl was used as a nonspecific control. We found that activation of Wnt signaling by treatment with LiCl completely restored differentiation in JARID2-depleted cells as assayed by immunohistochemistry for MHC (Fig. 4a). RNA analysis also showed a restoration of differentiation-specific gene expression including Tnni2 mRNA (Fig. 4b) and Mylpf mRNA (Fig. 4c).
We next asked whether expression of MYOD and MYOG was restored upon activation of Wnt. We found that the mRNA expression of Myod1 (Fig. 5a) and Myog (Fig. 5b) was restored at the RNA level following LiCl treatment. The activation of MYOD and MYOG was confirmed at protein expression level (Fig. 5c). We sought to confirm our results using an expression construct for Wnt3a (cDNA) . We found that ectopic WNT3A promoted the expression of Myog mRNA in JARID2 depleted cells (Fig. 5d). RNA expression of the differentiation-specific markers Mylpf, Tnni2 and Lmod2 was also activated by WNT3A in JARID2-depleted cells (Fig. 5e). As an additional confirmation of the block to Wnt signaling in these cells, we also used the TOPFlash/FOPFlash luciferase reporter system. We found that TOPFlash, which contains intact TCF-binding sites, was more active than FOPFlash, which contains mutated TCF-binding sites, in scr control cells. However, in cells depleted for JARID2, TOPFlash was not activated. Treatment of shJarid2 cells with LiCl restored activation of TOPFlash. Transfection of Wnt3a (cDNA) also robustly restored TOPFlash activation (Fig. 5f). Taken together, these data strongly support a block to Wnt signaling in the absence of JARID2.
To understand how Wnt signaling was blocked in JARID2-depleted cells, we examined the expression of the downstream mediator of canonical Wnt signaling, β-catenin (Ctnnb1). Analysis of the RNA expression level of Ctnnb1 showed no significant deregulation (Fig. 6a). Protein expression in total cell extracts also showed no significant change of β-catenin expression in JARID2-depleted cells (Fig. 6b). While we saw no obvious change in total β-catenin expression in JARID2-depleted cells, we next asked whether β-catenin localization was altered in these cells. Nuclear and cytoplasmic fractions of JARID2-depleted cells with and without LiCl treatment were probed for β-catenin. In control cells, we found that β-catenin was translocated to the nucleus upon differentiation, resulting in an increased level of nuclear β-catenin (Fig. 6c). This increase was not seen in JARID2-depleted cells, which showed reductions in nuclear β-catenin (Fig. 6c). A corresponding increase in cytoplasmic β-catenin could be seen in the JARID2-depleted cells upon differentiation, further supporting the block to β-catenin translocation (Fig. 6d). However, treatment with LiCl restored the translocation of β-catenin to the nucleus in JARID2-depleted cells (Fig. 6c). A corresponding reduction in cytoplasmic β-catenin was also observed (Fig. 6d). We also examined the expression of GSK3β under these conditions and saw no significant change in the expression of GSK3β in JARID2 depleted cells with or without LiCl treatment (Additional file 3: Figure S3).
β-catenin has previously been reported to activate Myod1 , and we had observed that activation of Wnt in JARID2 depleted cells both activated MYOD and enhanced nuclear translocation of β-catenin to the nucleus. We next asked whether β-catenin was bound to the Myod1 promoter in JARID2-depleted cells following LiCl treatment using ChIP assays. Previous work has shown that β-catenin binds to the DRR of the Myod1 promoter . We found that β-catenin was bound to the DRR region of the Myod1 promoter in normal cells upon differentiation and that this binding was lost upon JARID2 depletion (Fig. 6e). However, treatment with LiCl restored the binding of β-catenin to the DRR region of the Myod1 promoter (Fig. 6e). These effects were specific to the DRR region and were not observed in PRR or CE regions (Fig. 6f, g). Our data show that β-catenin directly activates Myod1 under control of the JARID2/PRC2 complex.
To identify the molecular mechanism that could explain the block to Wnt signaling in JARID2 depleted cells, we next examined the mRNA expression of two Wnt antagonists, Sfrp1 and Nkd1. We found that Sfrp1 mRNA was highly upregulated when JARID2 was depleted (Fig. 7a). In contrast, Nkd1 mRNA showed no upregulation and in fact was downregulated upon JARID2 depletion (Additional file 4: Figure S4 a). We next asked whether activation of Wnt signaling could reverse the upregulation of Sfrp1. JARID2 depleted cells were treated with LiCl, and we found that activation of Wnt was sufficient to downregulate Sfrp1 mRNA (Fig. 7b). To determine whether Sfrp1 was a direct target of JARID2, we performed ChIP assays and found that JARID2 binds to the Sfrp1 promoter and depletion of JARID2 causes a loss of this enrichment (Fig. 7c). We also found that H3K27me3 was present at the Sfrp1 promoter in normal cells, indicating that it is normally repressed by the PRC2 complex (Fig. 7d). Upon JARID2 depletion, H3K27me3 was reduced on the Sfrp1 promoter (Fig. 7d), which demonstrates that the promoter methylation of H3K27 is dependent on JARID2. We also found that H3K27me3 was present on the Nkd1 promoter and depletion of JARID2 reduced H3K27me3 on this promoter (Additional file 4: Figure S4 b). However, our mRNA expression data show that the loss of H3K27me3 on the Nkd1 promoter is not sufficient for activation of this gene.
To determine whether SRFP1 was responsible for the differentiation block we observed, an expression construct for Srfp1 (cDNA) was cloned and transiently expressed in C2C12 cells. We confirmed that Sfrp1 mRNA could be detected in these cells (Fig. 7e). We also found that ectopic expression of SFRP1 was sufficient to inhibit the mRNA expression of Myod1 (Fig. 7f) and Myog (Fig. 7g). RNA expression of a differentiation marker, Tnni2, was also strongly inhibited in the presence of SFRP1 (Fig. 7h). The inhibition of MYOD and MYOG by SFRP1 was confirmed at the protein level (Fig. 7i). We also asked whether SFRP1 could inhibit Wnt transcriptional activity using the TOPFlash/FOPFlash luciferase reporter system and found that transfection with Sfrp1 (cDNA) robustly inhibited TOP activity (Fig. 7j). We also asked whether SFRP1 was responsible for the block to β-catenin nuclear translocation that we observed in JARID2 depleted cells. We found that ectopic expression of SFRP1 was sufficient to inhibit the nuclear translocation and increase the cytosolic localization of β-catenin (Fig. 7k). These data show that the repression of Sfrp1 by JARID2 and the PRC2 complex is essential for activation of the canonical Wnt pathway, which normally induces β-catenin translocation to the nucleus for the activation of Myod1.
To determine whether the repression of Sfrp1 by JARID2 and the PRC2 complex was relevant beyond skeletal muscle, we asked whether the expression of JARID2 was correlated with SFRP1 in the Cancer Genome Atlas (TCGA) data. We first investigated the overall survival correlation between the expression of JARID2 and SFRP1 using the OncoLnc tool as previously described . Interestingly, both the genes queried were highly ranked and showed opposite Cox coefficient signs, positive for JARID2 and negative for SFRP1, in several cancer types including skin cutaneous melanoma (SKCM) and breast invasive carcinoma (BRCA). SKCM was chosen for the analysis presented here, but BRCA showed very similar results (data not shown). The expression data (RSEM) and clinical data for all the patients (N = 459) from the TCGA database were downloaded using the OncoLnc tool. For the correlation analysis, five of the “extreme outlier” samples were removed (N = 454). We found that, as anticipated from our results, the expression of JARID2 and SFRP1 was negatively correlated (Fig. 8a). We then sought to understand the survival prognosis between two non-overlapping cohorts of patients divided based on the expression of each gene, with the top 25 percentile defined as “High” and lower 25 percentile defined as “Low” (Additional file 5: Figure S5 a and b). Using Kaplan–Meier analysis, we found that low JARID2 expression and high SFRP1 expression were associated with significantly higher overall survival over a 400-month interval (Fig. 8b, c). We further performed correlation analysis between the expression of JARID2 and SFRP1 between two groups divided based on JARID2 expression as used for the survival analysis. We found that the negative correlation between the expression of JARID2 and SFRP1 was strengthened (Additional file 5: Figure S5 c). All data obtained from the TCGA database and resulting statistical analysis are shown in Additional file 6: File S1.
Taken together, our results establish that JARID2 and the PRC2 complex are required for skeletal muscle differentiation by directly repressing the Wnt antagonist, Sfrp1. Repression of Sfrp1 allows Wnt signaling to activate Myod1 for myogenic differentiation to proceed (Fig. 9). The repression of Sfrp1 by JARID2 and the PRC2 complex clearly extends beyond skeletal muscle and has important implications for development, adult homeostasis and disease.
We show here that JARID2 and the PRC2 complex are required to initiate skeletal muscle differentiation by modulating the Wnt signaling pathway that is required to activate Myod1. The direct repression of Sfrp1 by JARID2 allows Wnt signaling to promote myogenesis. The role of Wnt signaling during myogenesis is complex, but it is well established that Wnt signaling is essential for embryonic muscle development and adult muscle regeneration (reviewed in ). Canonical Wnt signaling has been shown to be required for the differentiation of myoblasts . The importance of canonical Wnt signaling in myogenesis has been demonstrated through series of loss- and gain-of-function assays that showed loss of β-catenin delayed differentiation, while constitutive overexpression of β-catenin results in precocious differentiation . However, the molecular pathways controlling this process have not been fully characterized. Recent work has shown that the methyltransferase, SETD7, enhances skeletal muscle differentiation by priming β-catenin for nuclear import, thus enabling Wnt signaling . Wnt signaling controls myogenic differentiation in part by regulating expression of the MRFs, including MYOD and MYF5 [2, 30,31,32]. However, the molecular mechanisms by which the Wnt pathway controls MRF expression have remained to be elucidated.
Our data provide more evidence of the essential role that Wnt signaling plays in myogenesis, but also expands the understanding of the roles of JARID2 and the PRC2 complex in skeletal muscle. The PRC2 complex has been shown to be required for satellite cell proliferation , but our results show that this complex is also critical for the transition to the terminal differentiation stage of myogenesis. Importantly, we also show here that JARID2 is required to guide the PRC2 complex to many of the previously described gene targets of the PRC2 complex, including Myog . The impaired expression of MYOD and MYOG observed upon depletion of JARID2 or EZH2 was highly surprising based on previous studies. We have shown that depletion of JARID2 modestly relieves the potent suppression of Myog mRNA expression by exogenous CIITA . Enhanced EZH2 expression has been shown to inhibit differentiation . In this study, an siRNA approach with EZH2 led to a decrease in promoter methylation of differentiation-specific genes. Significantly, while decreases in promoter methylation at genes such as Mck were observed upon EZH2 depletion, a corresponding increase in protein expression was not observed, consistent with our work. Other studies have also observed the anticipated increased expression of myogenin and downstream targets upon Suz12 depletion, but this study utilized a transient siRNA approach . We show here that a transient approach using the shRNA constructs characterized here also results in a transient upregulation of Myog mRNA, indicating that temporal and overall level of EZH2 depletion will lead to differential effects, complicating any comparison of the results. The data suggest that the level of EZH2 is precisely controlled in skeletal muscle cells and positive or negative alterations to that level leads to deleterious effects and differential experimental outcomes. A conditional ablation of Ezh2 in satellite cells using a Pax7-Cre driver showed an increase in the MYOG-positive satellite cell pool, indicating precocious differentiation, but a delay in MYOG-positive cells following injury . Other studies have shown that siRNA against EZH2 in isolated satellite cells leads to a downregulation of myogenin and inhibition of EZH2 in these cells leads to decreases in downstream targets such as MCK . These results highlight the complexity of the role of EZH2 in skeletal muscle. Additional studies have shown that depletion of JARID2 mRNA  or inhibition of EZH2  led to increases in MYOG mRNA and differentiation-specific gene expression in rhabdomyosarcoma (RMS) cell lines. Any comparison with RMS cell lines is complicated by the fact that RMS cells are shown to overexpress JARID2 and PRC2 proteins and, yet, highly express MYOD and MYOG and still fail to differentiate. A repressive basic helix-loop-helix factors, musculin (MSC), antagonize the activity of MYOD in myoblasts and in rhabdomyosarcoma [36, 37]. Additionally, Wnt signaling is known to be deregulated in RMS cells [38, 39], which is likely to contribute to the significant differences we observe in C2C12 cells. Intriguingly, SFRP3 has been shown to be required for PAX3-FOXO1-mediated tumorigenesis , again highlighting the deregulation of Wnt signaling in these cells.
The molecular explanation of our surprising result that JARID2 and EZH2 were required to activate MYOD in normal cells was suggested by studies using high-throughput techniques that showed that JARID2 and the polycomb (PcG) group of proteins have a large number of overlapping targets in embryonic stem (ES) cells, which included several Wnt signaling pathway genes and suggested a possible regulation of the Wnt signaling pathway by JARID2 [13, 41]. Additionally, the loss of JARID2 in murine ES cells was shown to result in the constitutive overexpression of Nanog mRNA and a reduction in the activity of β-catenin along with a downregulation of Wnt/PCP activity . In this study, a panel of Wnt antagonists, including Sfrp1, was found to be de-repressed, although no change in H3K27me3 on the Sfrp1 promoter was observed in Jarid2 null cells, suggesting that the JARID2 regulation of Sfrp1 was independent of PRC2 in mES cells . Interestingly, one of the Wnt antagonists included in the previous study, NKD1, was shown to be directly regulated by the PRC2 complex in hepatocellular carcinoma . In our study, while we observed a decrease in H3K27me3 status on the promoter of Nkd1 upon depletion of JARID2, we saw no upregulation in mRNA expression. However, our identification of Sfrp1 as a direct target of JARID2 is highly significant as we establish here that not only is Sfrp1 a direct target of JARID2 and the PRC2 complex in skeletal muscle cells, but that SFRP1 is sufficient to recapitulate the Wnt signaling block observed in JARID2 depleted cells.
Our work supports a growing body of evidence showing an essential role for JARID2 during development. JARID2 has previously been shown to be required for heart development . However, in heart development, JARID2 functions in association with H3K9 methyltransferases including SETD1, G9a and GLP [22, 43,44,45]. In contrast, at the majority of targets we studied in C2C12 cells, we did not see any significant changes in H3K9me status upon JARID2 depletion. Instead, we observed a significant change in H3K27me3 status at each of these genes, indicating a PRC2-dependent function of JARID2. This is in agreement with previous studies that have shown the role of JARID2 in targeting the PRC2 complex to target genes [11, 13, 19, 41]. However, earlier studies with ES cells showed that loss of Jarid2 delayed ES cell differentiation, while loss of PRC2 enhanced differentiation . This was attributed to the repressive role of JARID2 on PRC2 complex activity. In our study, we find that depletion of JARID2 or EZH2 inhibits skeletal muscle differentiation through co-regulation of Sfrp1 and the downstream regulation of the MRFs required for differentiation. Our data do not exclude a yet uncharacterized role of JARID2 in guiding H3K9 methyltransferases in skeletal muscle, and this remains to be further explored in future studies.
The data showing that JARID2 and SFRP1 are inversely correlated in melanoma are intriguing and suggest that JARID2 and PRC2’s modulation of Wnt signaling is highly significant for many cellular systems. EZH2 is known to be highly expressed in many cancers (reviewed in ), and elevated levels of JARID2 have been implicated in several cancers as well [34, 47,48,49]. Deregulated Wnt signaling has also shown to be correlated with poor survival in many cancer studies (reviewed in ). In this study, we provide evidence on the direct relationship between epigenetic modifiers, JARID2 and the PRC2 complex, and a canonical Wnt signaling regulator, SFRP1, that are significantly correlated in cancer patients and significantly associated with the lifespan and overall survival of these patients. The correlation further strengthens the molecular mechanism we discovered using an in vitro cell system. In addition to SKCM, multivariate Cox regression analysis using OncoLnc showed that overall survival of the patients was also correlated in four other cancer types in a similar fashion as shown for SKCM, including breast invasive carcinoma (BRCA). Thus, the relationship discovered in this study between JARID2 and the PRC2 complex and the Wnt signaling pathway goes beyond myogenesis. Uncovering how JARID2 and SFRP1 modulate cancer progress and patient survival in these cancers will be an important future direction of this study. Several therapeutic agents targeting the Wnt pathway have entered clinical trials , and EZH2 inhibitors are also entering clinical trials . Wnt and EZH2 targeting agents have shown clinical promise, but it has been noted that caution must be taken with these agents as both pathways are also indispensable for development processes and adult homeostasis. Precisely understanding the molecular mechanisms utilized in both normal and cancer cells will be required to effectively employ these novel therapeutic approaches.
We show that, like EZH2, JARID2 is expressed in proliferating myoblasts, but downregulated upon differentiation. JARID2 guides the PRC2 complex to many of the known PRC2 targets in skeletal muscle. We also reveal the unexpected finding that JARID2 and PRC2 are required to initiate skeletal muscle differentiation by modulating Wnt signaling through the direct repression of Wnt antagonists.
C2C12 cells (ATCC) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (HyClone) according to standard protocols. Proliferating C2C12 myoblasts were grown in DMEM supplemented with 10% fetal bovine serum (HyClone). To induce differentiation of C2C12 myoblasts into myotubes, cells were grown to 90% confluence and the media was switched to DMEM supplemented with 2% horse serum (HyClone). C2C12 cells were grown in differentiation medium for the number of days indicated in each experiment. Primary myoblasts were isolated according to standard protocols .
shRNA knock down
Jarid2 was depleted with shRNA constructs (pLOK.1, Sigma) as previously described . Ezh2 knock down lines were constructed with shRNA constructs designed by the RNAi Consortium in the pLOK.1 plasmid (Open Biosystems). Three constructs targeting murine Jarid2 and Ezh2 mRNA, and one scrambled control were linearized, transfected into C2C12 cells, and selected with puromycin (2 μg/ml). Individual clones were selected and propagated.
C2C12 cells were transfected with TurboFect (Fisher Scientific) according to manufacturer’s protocol. The plasmid pEMSV-MyoD (provided by Andrew Lassar, Harvard Medical School) was used for expressing MYOD. Active Wnt3A-V5 was a gift from Xi He (Addgene plasmid #43810) . All transfections were performed in three technical replicates, and a minimum of three biological replicates were performed for each experiment.
To clone murine genes, total RNA from C2C12 cells was extracted with Trizol (Invitrogen) and used to generate cDNA through the use of the Superscript III First Strand Synthesis System for RT-PCR kit with oligo dT primers (Invitrogen). Murine SFRP1 was amplified from cDNA generated from undifferentiated C2C12 cells using primers corresponding to the coding regions with engineered restriction sites. Primers are described in Additional file 7: Table S1. The resulting PCR product was cloned using restriction enzymes NotI and XhoI and the pCMV-tag1 cloning vector (Agilent). All resulting clones were confirmed by sequencing.
TOPFlash/FOPFLash luciferase assays
C2C12 cells were transfected with TurboFect (Fisher Scientific) according to standard protocols. M50 Super 8x TOPFlash (Addgene plasmid # 12456) and M51 Super 8x FOPFlash (TOPFlash mutant) (Addgene plasmid # 12457) were gifts from Randall Moon . Luciferase activity was determined using the Dual-Luciferase Reporter Assay System (Promega). C2C12 cells were seeded at a density of 2 × 103 cell per well in 96-well plates and transfected with 0.4 μg of DNA. Transfections were normalized to Renilla luciferase. Transfections were performed in triplicate, and all data sets were repeated three times.
Western blot analysis
Cell extracts were made by lysing phosphate-buffered saline (PBS)-washed cell pellets in radio-immunoprecipitation assay buffer (RIPA) supplemented with protease inhibitors (Complete protease inhibitor, Roche Diagnostics). Following incubation on ice, clear lysates were obtained by centrifugation. Protein concentrations were determined by Bradford’s assay (Bio-Rad). For each sample, 30 µg of protein was loaded on each gel. Proteins were transferred onto a PVDF membrane using a tank blotter (Bio-Rad). The membranes were then blocked with 5% nonfat milk in 1X Tris-buffered saline plus Tween 20 (TBST) and incubated with primary antibody overnight at 4 °C. Membranes were then washed with 1X TBST and incubated with the corresponding secondary antibody. Membranes were again washed with 1X TBST, incubated with chemiluminescent substrate according to manufacturer’s protocol (SuperSignal, Pierce) and visualized by autoradiography. The antibodies used include anti-Jarid2 (Cell Signaling), anti-Ezh2 (Cell Signaling), anti-myogenin (F5D, Developmental Studies Hybridoma Bank (DSHB)), anti-MyoD (C-17, Santa Cruz Biotechnology (SCBT)), anti-β-catenin (E5, SCBT), anti-MHC (MF-20, DSHB), anti-Lamin A/C (E-1, SCBT), anti-TBP (Cell Signaling), anti-GAPDH (Millipore) and anti-GSK3β (0011-A, SCBT). Densitometry to quantify protein expression levels was performed with ImageJ software (NCBI) on at least three independent experiments. Representative autoradiography films are shown.
Quantitative real-time PCR
RNA was isolated from cells by Trizol extractions (Invitrogen). Following treatment with DNase (Promega), two micrograms of total RNA was reverse transcribed with MultiScribe™ MuLV reverse transcriptase (Applied Biosystems). cDNA equivalent to 40 ng was used for quantitative polymerase chain reaction (qPCR) amplification (Applied Biosystems) with SYBR green PCR master mix (Applied Biosystems). Samples in which no reverse transcriptase was added (no RT) were included for each RNA sample. qPCR data were calculated using the comparative Ct method (Applied Biosystems). Standard deviations from the mean of the [Δ] Ct values were calculated from three independent RNA samples. Primers are described in Additional file 7: Table S1. Where possible, intron-spanning primers were used. All quantitative PCR was performed in three technical replicates and three independent RNA samples representing biological replicates were assayed for each time point. For measurements of relative gene expression, a fold change was calculated for each sample pair and then normalized to the fold change observed at HPRT and/or 18S rRNA.
Chromatin immunoprecipitation assays
ChIP assays were performed as described previously . The following antibodies were used: anti-Jarid2 (Cell Signaling), anti-H3K27me3 (Cell Signaling), anti-pan methyl H3K9 (D54, Cell Signaling) and anti-β-catenin (E-5, SCBT). Rabbit IgG (SCBT) was used as a nonspecific control. Primers are described in Additional file 7: Table S1. The real-time PCR was performed in three technical replicates. The results are represented as percentage of IP over input signal (% Input). All ChIP assays shown are representative of at least three individual biological replicate experiments. Standard error from the mean was calculated and plotted as error bar.
TCGA (the cancer genome atlas) data analysis
The “OncoLnc” tool was used to download patient information on skin cutaneous melanoma (SKCM) from The Cancer Genome Atlas (TCGA) database: http://cancergenome.nih.gov/, as described . Using Kaplan–Meier analysis, survival plots were generated for non-overlapping top 25 percentile (high) against bottom 25 percentile (low) patient groups divided according to the mRNA expression level of JARID2 and SFRP1. The expression data downloaded from TCGA for all the patients were used for Pearson’s correlation test between JARID2 and SFRP1, and scatterplots were generated. Student’s t test was performed between non-overlapping high- and low-group patients for JARID2 and SFRP1 expression level and was plotted as box plots. All the statistical analysis and graphs were made using SPSS Statistic 24.0.0 software.
Data are presented as means ± standard errors (S.E.). Statistical comparisons were performed using unpaired two-tailed Student’s t tests or one-way ANOVA followed by Tukey’s HSD (honest significant difference) post hoc test. For one-way ANOVA analysis, all means not sharing the same letter were statistically significant. Probability value of < 0.05 was taken to indicate significance.
wingless-related integration site
histone H3 lysine 27
histone H3 lysine 9
short/small hairpin RNA
short interfering RNA
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AA performed the experiments. AA and JD designed the experiments and wrote the paper. Both authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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Adhikari, A., Davie, J. JARID2 and the PRC2 complex regulate skeletal muscle differentiation through regulation of canonical Wnt signaling. Epigenetics & Chromatin 11, 46 (2018) doi:10.1186/s13072-018-0217-x