Fig. 2

Loss of EZH2 also inhibits C2C12 differentiation. a Ezh2 mRNA was depleted using stable cell lines expressing control scramble shRNA (scr) or individual Ezh2-specific shRNA (shEzh2) constructs. Depletion was confirmed by analysis of mRNA by qRT-PCR. Error bars are S.E.M. **p value < 0.01 and ***p value < 0.001 versus scr. n ≥ 3. b EZH2 protein depletion was confirmed by western blot assays. c Differentiation is impaired upon EZH2 depletion. Cells were differentiated for 2 days for immunohistochemistry with antibodies against myosin heavy chain (MHC). Nuclei were stained with DAPI. Images were taken at × 400 magnification. Bars represent 100 μm. d mRNA of differentiation-specific genes as indicated was downregulated in EZH2 depleted cells assayed by qRT-PCR. Error bars are S.E.M. **p value < 0.01 versus time-matched scr. n ≥ 3