- Open Access
Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1)
© Buro et al; licensee BioMed Central Ltd. 2010
- Received: 6 May 2010
- Accepted: 8 September 2010
- Published: 8 September 2010
Signal transducer and activator of transcription (STAT) activation of gene expression is both rapid and transient, and when properly executed it affects growth, differentiation, homeostasis and the immune response, but when dysregulated it contributes to human disease. Transcriptional activation is regulated by alterations to the chromatin template. However, the role of histone modification at gene loci that are activated for transcription in response to STAT signaling is poorly defined.
Using chromatin immunoprecipitation, we profiled several histone modifications during STAT1 activation of the interferon regulatory factor 1 gene (IRF1). Methylated lysine histone proteins H3K4me2, H3K4me3, H3K79me3, H3K36me3 and monoubiquitinated histone ubH2B are dynamic and correlate with interferon (IFN)γ induction of STAT1 activity. Chemical inhibition of H3K4 methylation downregulates IRF1 transcription and decreases RNA polymerase II (Pol II) occupancy at the IRF1 promoter. MEN1, a component of a complex proteins associated with Set1 (COMPASS)-like complex and the hBRE1 component, RNF20, are localized to IRF1 in the uninduced state and are further recruited when IRF1 is activated. RNAi-mediated depletion of RNF20 lowers both ubH2B and H3K4me3, but surprisingly, upregulates IFNγ induced IRF1 transcription. The dynamics of phosphorylation in the C-terminal domain (CTD) of Pol II are disrupted during gene activation as well.
H2B monoubiquitination promotes H3K4 methylation, but the E3 ubiquitin ligase, RNF20, is repressive of inducible transcription at the IRF1 gene locus, suggesting that ubH2B can, directly or indirectly, affect Pol II CTD phosphorylation cycling to exert control on ongoing transcription.
- Histone Modification
- H3K4 Methylation
- 2fTGH Cell
- RNF20 cDNA
- Polymerase Associate Factor Complex
In response to a variety of extracellular ligands, signal transducers and activators of transcription (STATs) are rapidly recruited from their latent state in the cytoplasm to cell surface receptors, where they are phosphorylated by tyrosine kinases. They then translocate to the nucleus, bind DNA response elements and drive the transcription of target genes, affecting growth, differentiation, homeostasis and the immune response . Not surprisingly, given their widespread involvement in normal cellular processes, dysregulation of STAT activity contributes to human disease, particularly to cancers. Persistently active STAT3 and STAT5 are present in breast cancers, head and neck cancers, prostate cancers, multiple myeloma, leukemias and lymphomas [2, 3].
STAT activation is both rapid and transient, with the downregulation of STAT activity achieved by several mechanisms, including dephosphorylation by the 45-kDa nuclear phosphatase T cell protein tyrosine phosphatase (TC45), which inactivates the STATs by removing their required tyrosine phosphates, and the cytoplasmic phosphatase Src homology region 2 domain-containing phosphatase-1 (SHP-1), which dephosphorylates the kinases upstream of STATs. Other negative regulators include the suppressor of cytokine signaling (SOCS) family of proteins, which are induced by cytokine signaling and STAT activation and participate in a negative feedback loop, and the protein inhibitor of activated STAT (PIAS) family of proteins, which can directly inhibit STATs by preventing their DNA binding .
Transcriptional activation, like that mediated by STATs, is one of several nuclear processes regulated by alterations to the chromatin fiber. Such alterations are dynamic and include covalent histone modifications and DNA methylation, as well as the activities of ATP-dependent complexes . Chromatin's core structure, the nucleosome, is composed of 146 bp of DNA wrapped around an octamer of histone proteins (H3, H4, H2A and H2B). A number of post-translational modifications to the nucleosome, mostly in histone N-terminal tails, have been described, including methylation, acetylation, phosphorylation and ubiquitination. These covalent modifications define the functional state of chromatin via both cis and trans mechanisms. Cis mechanisms, best typified by acetylation/deacetylation, result in changes to nucleosome packing that increase or decrease DNA accessibility. In trans mechanisms, non-histone proteins that possess particular binding domains recognize specific histone modifications and recruit additional factors that regulate chromatin structure [6, 7]. Together, cis and trans mechanisms embellish the chromatin fiber to generate biological effects that extend beyond the DNA sequence alone.
Several genome-wide studies have investigated how particular histone methylations correlate with gene expression in human cells [8–14]. Dimethylation and trimethylation of lysine 4 (H3K4me2, H3K4me3) in the N-terminal tail of histone H3 are known to correlate with an active chromatin state. Trimethylation of lysine 27 (H3K27me3) is associated with silenced chromatin, while monomethylation of this same residue is broadly localized to euchromatin. H3K36me3 is enriched downstream of the promoters of actively transcribed genes, and thus, is strongly correlated with H3K4me3, but is not correlated at all with H3K27me3. H3K9me3 is typically associated with transcriptional repression, as is H4K20me1  although others have linked these modifications to the activation of some genes [9, 16, 17]. H3K79me3 is associated with actively transcribed genes but Barski et al. found this modification is enriched at some silent genes [9, 18, 19]. Such discrepancies suggest that the function of histone methylation might be gene specific and depend upon the recruitment of different trans-acting complexes, or that methylation status might be dynamic and titrated during gene expression by the concerted activity of histone methyltransferases (HMTs) and demethylases (HDMs) [20–23].
In mammalian cells, there are multiple H3K4 methyltransferases, including SET1A/B and mixed lineage leukemia (MLL) proteins 1-4, which contribute to complex proteins associated with Set1 (COMPASS) and COMPASS-like complexes, respectively . WDR82, the human homolog of Cps35/Swd2, is associated only with SET1A/B complexes (not MLL1-4 complexes). Like Cps35, WDR82 interacts with chromatin in a manner that depends upon the monoubiquitination of H2B , suggesting a mechanism similar to the crosstalk mechanism that is well established in Saccharomyces cerevisiae where H3K4 methylation requires H2B monoubiquitination (ubH2B). Along these same lines, in vitro transcription assays suggest that the presence of ubH2B on chromatin stimulates SET1/COMPASS-dependent H3K4 dimethylation and trimethylation . It was reported recently that ubH2B is associated with the transcribed regions of highly expressed genes in human cells. However, RNF20/BRE1A, a human homolog of the yeast E3 ubiquitin ligase, Bre1, can both activate and repress distinct subsets of genes [27, 28].
While little is known about the role of histone modification in regulating the activity of genes that are poised for transcription in response to STAT signaling, the rapid and transient nature of STAT-triggered transcription makes it an attractive system in which to study dynamic signaling to chromatin. Interferon (IFN)γ activation of STAT1 drives higher order chromatin remodeling at the major histocompatability complex (MHC) and correlates with recruitment of the chromatin-remodeling enzyme BRG and histone acetylation [29, 30]. However, surprisingly, histone deacetylase activity is reportedly required for the transcription of IFN-responsive genes, but the mechanistic basis for this requirement is unknown [31, 32].
Here we report the detailed profiling of the changes in the histone modification landscape that accompany STAT1-activated expression of the interferon regulatory factor 1 gene (IRF1). To begin to address the molecular basis for these changes, we identified a COMPASS-like complex as the likely H3K4 methyltransferase associated with the IRF1 promoter and showed that full H3K4 trimethylation depends upon the activity of the ubiquitination enzyme RNF20/BRE1A. Furthermore, our data indicate that H2B monoubiquitination, located across the IRF1 gene, represses transcription when STAT1 signaling is induced by IFNγ.
Histone modifications correlate with inducible STAT1 activity
H3K79me3 overlaps with H3K4me2 but extends further into the coding region, consistent with its described association with transcriptional elongation (Figure 2e) . H3K79me3 is found only at very low levels at the IRF1 gene in 2fTGH cells in the uninduced state or in U3A cells (Figure 3). Thus, this modification specifically correlates with STAT1-induced transcription. This profile for H3K79me3, is different from the profile reported in a genome-wide study of CD4+ T cells which found H3K79me3 higher at silent promoters than at active ones, except in a narrow region near the TSS .
As expected, H3K36me3 was found to be biased toward the 3' region of the gene and is associated with elongation (Figure 2f). The H3K36me3 profile differs from the other profiles collected; specifically, this modification returns to uninduced levels at 5 h post-IFNγ treatment suggesting that a demethylase may target H3K36. The overall histone occupancy observed, determined using a pan H3 antibody, was consistent across the IRF1 locus, except at the TSS where a dip was observed as a result of TSS histone depletion (Figure 2g).
To confirm the dynamic nature of histone modification in STAT induced transcription, we performed ChIP assays at three other IFNγ responsive genes (RING4, PSMB8, GBP1) with quantitative real-time PCR (Q-PCR) primers designed to amplify the 5', 3' and middle regions of these gene loci and found similar patterns (data not shown). Taken together, the patterns and dynamics of these histone modifications indicate that STAT1 dependent genes experience transcription initiation but that downstream events, dependent upon STAT1's activation and DNA binding, regulate the accumulation of transcripts.
Inhibition of methyltransferase activity decreases H3K4me3 and H3K36me3 at the IRF1 gene as well as IRF1 transcription
MTA has also been shown to decrease HSV-1 gene transcription during lytic infection . Therefore, we considered if the decreases we observed for H3K4me3 and H3K36me3 correlated with decreased transcription of the IRF1 gene using reverse transcriptase followed by Q-PCR. Indeed, an approximately twofold reduction in the steady-state level of IRF1 mRNA was observed (Figure 4d). We confirmed that the reduction was due to decreased transcription by quantitating the IRF1 transcript levels in heterogeneous nuclear RNA (hnRNA) using PCR primers that target intronic sequences (Figure 4e) . STAT1 occupancy was not altered by MTA treatment, but Pol II occupancy at the TSS was decreased (Figure 4g, h).
RNF20 and a MLL/Menin complex are localized to IRF1
There are several recognized H3K4 methyltransferases in mammalian systems, including SET1A/B and MLL1-4 . However, ChIP-grade antibodies that discriminate among all of them are not available. Because the non-enzymatic proteins that contribute to COMPASS and COMPASS-like complexes do show some specificity, we used a ChIP-grade antibody against Menin (MEN1), which is a component unique to the MLL1 and MLL2 COMPASS-like complexes. ChIP assays showed that MEN1 is constitutively associated with the IRF1 promoter and that induction leads to an increase in a MEN1-containing complex (Figure 6b). The COMPASS-like activity constitutively associated with the IRF1 promoter, potentially explains the significant amounts of H3K4 methylation observed in uninduced cells and even in U3A cells (Figures 2 and 3).
RNAi-mediated knockdown of RNF20 upregulates IRF1 transcription
H2B monoubiquitination and H3 lysine methylation
Supportive of a crosstalk mechanism where ubH2B promotes H3K4 methylation, we note that inhibition of H2B ubiquitination using carbobenzoxy-L-leucyl-L-leucyl-L-leucinal (MG132), a proteosome inhibitor that has been used to rapidly and efficiently deplete ubH2B [27, 41], correlated with loss of both H3K4me3 and H3K4me2 (Additional file 2b, c) in response to IFNγ, while H3K36me3 was unaffected (Additional file 2d). When 2fTGH cells were treated with MG132, ubH2B was eliminated globally (Additional file 1d), and at the IRF1 gene locus when cells were induced with IFNγ (Additional file 2a).
Genetic studies in yeast and mammalian in vitro transcription assays have demonstrated that H2B monoubiquitination depends upon the early steps of transcriptional elongation, requiring the presence of the polymerase associated factor (PAF) complex and the addition of nucleoside triphosphates (NTPs), and not simply recruitment of Rad6/Bre1 [26, 38]. To find out if ubH2B at the IRF1 gene also depends upon elongation, 2fTGH cells were treated with the elongation inhibitor 5,6-dichlorobenzimidazole riboside (DRB) [16, 23]. Pol II promoter occupancy is unaffected by DRB treatment . We confirmed that DRB ablates IRF1 induced gene expression. (Additional file 3a) and it also significantly inhibits basal transcription. In ChIP assays, in the DRB treated condition, induced ubH2B was strongly decreased, as was H3K4me3 (Additional file 3b, c). H3K36me3 normally correlates with ongoing transcription and so, not surprisingly, induced H3K36me3 was also decreased by DRB (Additional file 3d).
RNAi-mediated knockdown of RNF20 alters Pol II C-terminal domain (CTD) phosphorylation
In yeast, H2B ubiquitination has been functionally tied to transcriptional elongation; H2B ubiquitination/deubiquitination occurs dynamically, with deubiquitination required for the recruitment of the RNA polymerase II CTD serine 2 kinase, Ctk1[39, 42]. Additionally, H3K4 methylation has been attributed a repressive role at the GAL10-GAL1 locus . H3K4me2/3 occurs via cryptic transcription and recruits a histone deacetylase (HDAC) activity (Rpd3) to dampen GAL1 promoter activity by inhibiting Pol II recruitment. In the absence of H3K4 methylation, GAL1 induction is accelerated.
In this study, we characterized several histone modifications in STAT1 activation of the IRF1 gene and showed that ubH2B promotes H3K4me3 during induction. Additionally, the enzymatic activities that produce these modifications, COMPASS-like and RNF20, are found at the IRF1 gene and are further recruited by induction. These data, along with the observation that methyltransferase inhibition hinders IRF1 transcription, suggested a positive regulatory role for these enzymes. Unexpectedly, the E3 ligase that targets H2B for ubiquitination, RNF20, acts as a repressor of transcription, ostensibly by altering the dynamic phosphorylation of the CTD of Pol II that determines its initiation, elongation and termination.
Mammalian ubH2B function
Other studies of ubH2B in inducible mammalian gene expression have suggested alternative roles for this modification, than the repressive role our data indicates. Using in vitro transcription assays with a recombinant, fully H2B-ubiquitinated chromatin template, Kim et al. observed that H2B ubiquitination does not affect p53 activated transcription, arguing that H2B ubiquitination is a consequence of transcription and it does not directly affect the function of the transcriptional machinery . In contrast, ubH2B reportedly enhances the rate of transcriptional elongation by Pol II in response to retinoic acid , and at HOX genes . The dynamics of H2B ubiquitination during transcriptional activation (Figure 5) and the observation that RNF20 knockdown increases inducible IRF1 transcription (Figure 7a, b), while its overexpression inhibits IRF1 (Figure 7e) all argue against the ideas that this modification occurs in the wake of Pol II or promotes Pol II passage at the IRF1 gene. It is possible that these disparities are simply related to the use of in vitro assays that differently or incompletely recapitulate the inducible transcription that occurs in vivo. Alternatively, the function of ubH2B in gene expression might be context dependent.
RNF20 as repressor in mammalian gene expression
Using gene expression microarrays, Shema et al. identified two cohorts of genes that are selectively up or downregulated by RNF20 depletion in HeLa cells . Based on our results, we speculate that these two cohorts of genes may have different barriers built into their chromatin, which contribute to regulation of their expression. For instance, the cohort of genes that is activated by RNF20, such as TP53 and the HOX genes [28, 47] may not require a repressive mechanism to be generated by ubH2B to regulate their transcription and, in those cases, ubH2B works to promote elongation. However, genes normally suppressed by RNF20 are likely to be poised for activation and undergo basal transcription, and therefore rely on H2B monoubiquitination as a check against inappropriately activated transcription. It is worth noting that Shema et al. report that this later cohort of genes is populated by several early response and proliferative genes, is enriched in Pol II and H3K4me3 (as is IRF1) as compared to RNF20 dependent and independent genes, and that many are constitutively 'on' but in a 'low gear' state, reflecting the unique features of these genes that poise them for rapid activation in response to signaling events.
Similarities to activated transcription at the GAL1 gene in Saccharomyces cerevisiae
The role of histone modification in inducible gene expression is best characterized at the GAL1 locus in Saccharomyces cerevisiae . At that gene, dimethylation and trimethylation of histone H3K4 depend upon monoubiquitination of H2B (ubH2B) at K123 in a mechanism involving the Rad6/Bre1 ubiquitination enzymes and the Set1/COMPASS methyltransferase complex . While the precise mechanism for this trans-tail communication is not fully established, a model has emerged whereby the H2B ubiquitin ligase, Bre1, and the E2 ubiquitin conjugase, Rad6, are recruited to promoters by interaction with activators, such as Gal4 . The enzymatic activity for the monoubiquitination of H2B, however, depends on further interactions with the PAF complex, the Bur complex and the phosphorylation of the CTD of Pol II at serine 5. H2B ubiquitination then recruits Cps35, (aka Swd2) an essential component of COMPASS, which leads to H3K4 dimethylation and trimethylation by the methyltransferase, Set1 [42, 48–51]. H3K4me3 recruits the SAGA complex, which contains the deubiquitinase, Ubp8. Deubiquitination of ubH2B allows Ctk1 to phosphorylate the CTD of Pol II at serine 2, which in turn recruits the methyltransferase for H3K36, Set2 . Thus, Ubp8 provides the opposing deubiquitinase activity that is required for transition to efficient transcriptional elongation at the inducible GAL1 gene. ubH2B's transitory profile at the IRF1 gene indicates that it might be removed by an opposing ubiquitin hydrolase, as it is in yeast. While other events, such as histone exchange or direct or indirect RNF20 inactivation, are also possible, studies in the lab are ongoing to determine if USP22 (the mammalian homolog to Ubp8) also deubiquitinates ubH2B. It seems a likely possibility it does, since USP22 is required for MYC activated transcription , but whether ubH2B directly blocks recruitment of a kinase (P-TEFb) responsible for serine 2 phosphorylation in CTD of Pol II is not known .
Several other important research questions are suggested by the data presented here. First, what is the mechanistic basis for the defect observed in Pol II CTD phosphorylation cycling and is this the direct cause of increased transcription observed in the RNF20 depleted cell line? Might H2B monoubiquitination also impact the function of CTD phosphatases (Fcp1 and Rtr1) as it does the kinase (Ctk1) across gene loci ? Second, what is the basis for RFN20's recruitment? Does STAT1 help to recruit hBRE1, as Gal4 and p53 do [38, 55]? What is the role of the PAF complex? And what is the precise nature of the MEN1-containing, COMPASS-like complex and which of its components functions as the translator in the crosstalk with hRAD6/hBRE1? WDR82 mediates the crosstalk between hSET1/COMPASS and the ubiquitination complex, but it is a component specific to hSET1 . While we cannot rule out the possibility that SET1/COMPASS, or another H3K4 methyltransferase, is also involved redundantly, the colocalization of MEN1 and RNF20 at the IRF1 gene is the first evidence of a possible interaction between COMPASS-like and hBRE1 in mammalian gene expression.
A different role for H3K36me3
Of the several histone modifications we profiled in this study, only ubH2B and H3K36me3 return to basal levels, mirroring the dynamics of STAT1 activation (Figures 2a, f and 5a) and IRF1 transcription (Figure 1). In yeast, H2B deubiquitination drives H3K36me2, which then works to prevent cryptic transcription by Pol II in the 3' end of genes by recruiting a histone deacetylase [56–58]. In addition, H3K36me2 levels do not parallel transcriptional output in a titratable fashion, but rather reflect one of two states: uninduced/basal transcription or induced transcription [59, 60]. We, and Edmonds et al. , observed this same profile for H3K36me3 at inducible genes in mammalian cell lines. However, mammalian H3K36me1/me2 levels are very low, and deletion of the Set2 homolog responsible for H3K36me3, SETD2, removes all H3K36me3, but with no consequence for proper transcription, leading Edmunds et al. to conclude that K36 methylation in mammalian transcription does not mirror its role in yeast. Our data support this conclusion, given that forced H2B deubiquitination, achieved with MG132 and RNF20 knockdown, did not alter H3K36me3.
While it is not yet clear what H3K36me3 contributes to mammalian inducible gene expression, recent research has revealed that H3K36me3 preferentially marks exons relative to introns and it has been proposed that H3K36me3 exon marking connects transcription and splicing [61, 62]. While the primer pairs used to profile the IRF1 gene were designed without considering intron-exon structure, the exons are heavily biased to the 3' end in the IRF1 gene (Figure 2h). The 3' bias observed for H3K36me3 may reflect this.
Signal transduction pathways, like the JAK-STAT pathway, relay signals from the extracellular environment through the cytoplasm and ultimately to the DNA, which is organized as chromatin in the nucleus. Chromatin then serves as the template for dynamic nuclear signaling events (that is, post-translational modifications of histones) that regulate transcription. These dynamic signaling events are highly integrated, and it is becoming more clear that it is the proper balance between opposing enzymatic activities (E3 ubiquitin ligase/ubiquitin hydrolase, HMT/HDM, kinase/phosphatase and so on) that determine the functional output of a histone modification as either activating or repressing . Therefore, it is important to better define the function of histone modifications and the interplay among the enzymatic activities (COMPASS-like, hBRE1, PAF1 complex, USP22, and so on) that promote these modifications if we are to fully understand how chromatin contributes to both normal and aberrant activated transcription in mammalian systems.
The antibodies used were as follows: H3K4me3 (Abcam, Cambridge, MA, USA, ab8580), H3K4me2 (Millipore, Billerica, MA, USA, 07-030), H3K36me3 (Abcam ab9050), H3K79me3 (Abcam ab2621), Pan H3 CT (Millipore 07-690), ubH2B (Medimabs, Quebec, Canada, MM-0029-p), RNA Pol II (Santa Cruz, Santa Cruz, CA, USA sc-899), RNA polymerase II CTD repeat YSPTSPS (phospho S2) (Abcam ab5095), RNA polymerase II CTD repeat YSPTSPS (phospho S5) (Abcam ab5131), IgG (Jackson Immunoresearch, West Grove, PA, USA), STAT1 (Santa Cruz sc-345X), phospho-STAT1 (Tyr701) (Cell Signaling Technology, Danvers, MA, USA, 9171S), Menin (Bethyl Laboratories, Montgomery, TX, USA, A300-105A), RNF20 (Abcam ab70495), RNF20-ChIP-grade (Novus Biologicals, Littleton, CO, USA, NB100-2242), FLAG (Sigma), Anti-rabbit or anti-mouse horseradish peroxidase (HRP) (Jackson Immunoresearch).
Cell lines and chemical inhibitors
2fTGH and U3A cell lines were cultured in HyClone Dulbecco's modified Eagle medium (DMEM)/high glucose media supplemented with 10% cosmic calf serum and 10% antibiotic/antimycotic (Fisher Scientific, Pittsburgh, PA, USA). Interferon (IFN)γ treatment in all cases involved adding IFNγ (R&D Systems, Minneapolis, MN, USA, 5 ng/ml) to the media for 30 min, replacing with fresh media and harvesting cells at the indicated times. MTA (Sigma, St. Louis, MO, USA), MG132 (Calbiochem, San Diego, CA, USA), DRB (Sigma) treated cells were prepared as indicated in the figure legends.
Reverse transcriptase Q-PCR
Total RNA was collected using Isol-RNA lysis reagent (5 Prime, Gaithersburg, MD, USA). RNA was DNaseI (Invitrogen, Carlsbad, CA, USA) treated and phenol/chloroform extracted. RNA (2 μg) was converted to cDNA using the High Capacity RNA-to-cDNA kit (Applied Biosystems, Carlsbad, CA, USA). cDNA was then subjected to Q-PCR (Sybr Green, 7500 FAST Real Time PCR System, Applied Biosystems) using gene specific primers to the intronic or exonic region of the IRF1 gene. In all cases, an RT(-) control confirmed no DNA contamination. Primer sequences will be provided upon request. PCR efficiency was determined for all primer pairs before their use. To ensure the statistical significance of differences reported in the RT-Q-PCR assays, standard errors were calculated for the multiplicates, and when SE bars did not overlap, a paired t test confirmed significance, P ≤ 0.05.
Western blot analysis
Cells were collected after various treatments and whole cell extract, prepared as described in , was subjected to SDS-PAGE (30 μg) and transferred to a nitrocellulose membrane. Immunodetection was performed using anti-pan H3 CT (1:25,000), anti-ubH2B (1:1,000), anti-H3K4me3 (1:500), anti-H3K4me2 (1:5,000), anti-RNF20 (1:5,000), anti-STAT1 (1:1,000), anti-pY-STAT1 (1:1,000). A HRP anti-species secondary antibody (1:10,000) was then applied and immunoreactive proteins were visualized using chemiluminescence reagent (Thermo Scientific, Pittsburgh, PA, USA). Histone acid extraction was carried out as described previously . Bands were quantified with ImageJ.
ChIP was performed as described previously . Briefly, 1 × 107 cells were fixed in 1% formaldehyde for 10 min followed by the addition of 0.125 M glycine. Cells were lysed using a douncer and the fixed chromatin was sheared by sonication. Chromatin was subject to centrifugation (13,000 rpm for 25 min at 4°C) and was then incubated overnight with various antibodies. Pan H3 and IgG were included in all ChIPs as positive and negative controls. Immunoprecipitation was carried out with protein A agarose/salmon sperm beads (Millipore). After washing, the chromatin was eluted from the beads and the cross links were reversed by heating at 65°C overnight. DNA was treated with RNase A and proteinase K (5 Prime), purified via phenol/chloroform extraction, precipitated with ethanol overnight and resuspended in TE buffer. Samples were analyzed by quantitative real time-PCR (Applied Biosystems) using gene specific primers designed to run the length of the IRF1 gene. Primer sequences provided upon request. PCR efficiency was determined for all primer pairs before their use. Data are expressed as percentage of input and all experiments were performed in duplicate, if not triplicate. Where indicated the data were normalized to the Pan H3 levels. To ensure the statistical significance of differences reported in the ChIP assays, standard errors were calculated for the multiplicates and, if necessary, a paired t test confirmed significance, P ≤ 0.05.
Transfection of shRNAmir and expression vectors
A pGIPZ shRNAmir vector targeting RNF20 mRNA (RHS4430-98486410) as well as a non-silencing shRNA vector (RHS4346) were purchased from Open Biosystems, Huntsville, AL, USA. C-terminally FLAG tagged RNF20 was PCR cloned between the Kpn I and Eco RV sites of pcDNA3.0 (Invitrogen) using an RNF20 cDNA as template (Open Biosystems, MHS4426-98361130). Transfection of 2fTGH cells was carried out using Arrest-in reagent according to the manufacturer's protocol (Open Biosystems). Puromycin was used to select for stable shRNAmir cell lines and individual clones were characterized according to their RNF20 protein expression using western blotting.
We thank James Darnell (Rockefeller University) for 2fTGH and U3A cell lines, and Patrick Grant (University of Virginia) and Tom Milne (Rockefeller University) for helpful suggestions.
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