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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1)

Figure 2

Histone H3 lysine methylation across the interferon regulatory factor 1 gene ( IRF1 ) locus is dynamic in response to interferon (IFN)γ induction. (a-g) Chromatin immunoprecipitation (ChIP) of 2fTGH cells treated with IFNγ for 30 min, or uninduced, and then collected at various time points. The indicated antibodies were used and quantitative PCR (Q-PCR) quantified the precipitate yield, reported as percentage of input. IgG served as the negative control and Pan H3 antibody as the positive control and indicator of histone levels across IRF1 (g). Locations of Q-PCR primers designed to span the entire locus of IRF1 are shown in (h).

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Epigenetics & Chromatin

ISSN: 1756-8935