Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes
Epigenetics & Chromatin volume 9, Article number: 52 (2016)
The KMT2A/MLL1 lysine methyltransferase complex is an epigenetic regulator of selected developmental genes, in part through the SET domain-catalysed methylation of H3K4. It is essential for normal embryonic development and haematopoiesis and frequently mutated in cancer. The catalytic properties and targeting of KMT2A/MLL1 depend on the proteins with which it complexes and the post-translational protein modifications which some of these proteins put in place, though detailed mechanisms remain unclear.
KMT2A/MLL1 (both native and FLAG-tagged) and Msk1 (RPS6KA5) co-immunoprecipitated in various cell types. KMT2A/MLL1 and Msk1 knockdown demonstrated that the great majority of genes whose activity changed on KTM2A/MLL1 knockdown, responded comparably to Msk1 knockdown, as did levels of H3K4 methylation and H3S10 phosphorylation at KTM2A target genes HoxA4, HoxA5. Knockdown experiments also showed that KMT2A/MLL1 is required for the genomic targeting of Msk1, but not vice versa.
The KMT2A/MLL1 complex is associated with, and functionally dependent upon, the kinase Msk1, part of the MAP kinase signalling pathway. We propose that Msk1-catalysed phosphorylation at H3 serines 10 and 28, supports H3K4 methylation by the KMT2A/MLL1 complex both by making H3 a more attractive substrate for its SET domain, and improving target gene accessibility by prevention of HP1- and Polycomb-mediated chromatin condensation.
The human KMT2 proteins (formerly known as mixed-lineage leukaemia, MLL proteins) are highly conserved, SET domain-containing lysine methyltransferases, homologous to the Drosophila trithorax group proteins TRX (trithorax), TRR (TRX-related) and dSET1 [1, 2]. Like their Drosophila homologues, they maintain and enhance the activity of genes with key roles in differentiation and development , in part by regulating levels of histone 3 lysine 4 (H3K4) methylation, thereby, directly or indirectly, modifying chromatin structure [3, 4]. In humans, the KMT2 family comprises three pairs of paralogues, namely KMT2A and 2B (dTRX homologues, originally MLL1 and 2), KMT2C and 2D (dTRR homologues, originally MLL3 and 4) and KMT2F and G (dSET1 homologues, originally hSET1A and hSET1B). KMT2 family members are frequently mutated in leukaemias  and other cancers . In vivo, the KMT2 enzymes are found in multi-subunit complexes of which three proteins, WDR5, RbBP5 and ASH2L, provide a common stoichiometric core (reviewed in [1, 7]). These proteins enhance the weak catalytic activity of recombinant KMT2 by 50–500-fold [8, 9]. DPY30 also frequently associates with the core complex, providing a further twofold increase in methyltransferase activity . In addition to these common components, complexes built on each one of the three KMT2 subgroups can interact with a unique set of binding proteins. For example, only KMT2A/B complexes contain multiple endocrine neoplasia type 1 (MENIN)  and lens epithelium-derived growth factor (LEDGF) . These proteins seem to mediate interaction with transcription factors such as oestrogen receptor  and therefore influence gene targeting. In addition, KMT2 complexes have been shown to interact dynamically with a variety of transcription factors, including E2Fs  and p53  amongst several others (see [1, 7] for further details). It is likely that these variable protein associations make a major contribution to the catalytic properties and gene regulation profiles of the three KMT2 subgroups.
All KMT2 subgroups are specific for H3K4, but catalyse different methylation states. Recombinant KMT2A/B complexes give mono- and di-methylation (H3K4me1, H3K4me2), with only weak trimethylation [1, 15, 16]. KMT2C/D complexes give predominantly mono-methylation, whilst KMT2F/G complexes are capable of mono-, di- and trimethylation . The distinction is important, given that the different methylation states of H3K4 play different roles in transcriptional control. H3K4me1 is a consistent marker of regulatory enhancers [17, 18], whereas H3K4me3 is, amongst other things, a marker of active promoter regions [19, 20]. Various factors could contribute to these detailed, but functionally important, catalytic differences. For example, H2B ubiquitination enhances the activity of KMT2A/MLL1 and KMT2F, but not KMT2C . In addition, post-translational modification of individual subunits can influence catalytic properties; for example, SUMOylation of RbBP5 inhibits KMT2A/MLL1 by disrupting its association with ASH2L . These events may account for the occasional discrepancies between the catalytic properties of purified complexes and those reconstituted in vitro from recombinant proteins (see  for original references). Thus, much remains to be learned about the ways in which interacting factors and post-translational protein modifications determine the characteristic properties of particular KMT2 complexes. As the catalytic and structural requirements for the KMT2 complex are likely to be context dependent, varying from one cell type or developmental stage to another, any individual cell may contain a dynamic and variable population of complexes with different protein components.
The results presented here focus on the most widely studied mammalian KMT2, namely the TRX homologue KMT2A/MLL1. Human KMT2A is a large protein, 3963 amino acids which, uniquely within the KMT2 family, contains two sites that can be cleaved by the threonine aspartase Taspase 1 [22, 23]. (Its paralogue, KMT2B, contains only one such site.) Taspase 1 cleavage of newly translated KMT2A/MLL1 generates 320 kDa N-terminal and 180 kDa C-terminal fragments , which then form a non-covalent, heterodimeric complex. How this cleavage step contributes to KMT2A/MLL1 function remains uncertain , but it is likely that it allows the more effective configuration of the various functional domains spread across the protein. These include the lysine methyltransferase (SET) domain [3, 19]), and various protein-binding (PHD, bromo-domains ) and DNA-binding (CXXC, AT hook) domains [27, 28]). After post-translational processing, KTM2A/MLL1 is incorporated into a functional, multi-protein complex containing the four core components common to all KMT2 complexes (outlined above), plus a variable number of additional components . Some of these confer specific catalytic properties, including the lysine acetyltransferase CBP/KAT3A, which is necessary for the complex to activate transcription [30, 31], and MOF/KAT8, which specifically acetylates lysine 16 on histone H4, a modification known to decondense chromatin .
The association of histone acetyltransferases with KMT2A complexes and the effect of H2B ubiquitination on its methyltransferase activity exemplify the complex interactions (‘cross-talk”) of histone post-translational modifications (PTMs) as determinants of chromatin function. We have shown previously that global histone hyperacetylation induced by histone deacetylase inhibitors is accompanied, in at least some cell types, by increased methylation of H3K4 [33, 34]. In an attempt to explain this, we asked how PTMs might affect the ability of the H3 Tail to act as a methyltransferase substrate. In vitro assays using a recombinant SET domain methyltransferase and synthetic peptide substrates showed that H3 mono-, di- and trimethylation were maximally enhanced by acetylation of both H3K9 and H3K14, together with phosphorylation of H3S10 . Here, we address the in vivo significance of H3S10 phosphorylation for function of the KMT2A methyltransferase complex and examine the mechanism by which this modification is put in place. We show that mitogen- and stress-activated kinase, Msk1 (also known as RPS6KA5), is physically associated with the KMT2A/MLL1 complex in vivo and is necessary for the regulation of key KMT2A-regulated genes.
KMT2A and Msk1 are physically associated in various cell lines
The in vitro methylation of H3K4 by the KMT2A/MLL1 SET domain is strongly enhanced by phosphorylation of the histone peptide substrate at serine 10 , raising the possibility that phosphorylation of this residue may influence the gene regulatory activity of the KMT2A/MLL1 complex in vivo. Phosphorylation of H3S10 in chromatin can occur through the action of the closely related kinases Msk1 and Msk2 [35, 36], so we explored the possibility that Msk1 might operate as part of the KMT2A/MLL1 complex. Msk1 is constantly expressed in the cells but is active only after (auto-) phosphorylation and allosteric changes induced by ERK1 or 2 . The antibody used for these experiments is specific for Msk1 phosphorylated at S360, one of the last residues to become phosphorylated, and detects active Msk1 . In three different cell types—human lymphoblastoid cells (LCLs), mouse embryo fibroblasts (MEFs) and human embryonic kidney cells (HEKs)—material precipitated with antibodies to KMT2A contained readily detectable amounts of Msk1 (Fig. 1a). The protein often migrated as a double band, possibly due to allosteric changes and/or differences in phosphorylation status (Fig. 1a). Conversely, antibodies to Msk1 immunoprecipitated KMT2A/MLL1 (Fig. 1b, full-length blots from 1A and 1B are shown in Additional file 1). To validate this interaction, FLAG-tagged KMT2A/MLL1 was overexpressed in HEK293 cells and the associated complex purified (Fig. 1c). Western blot analysis of the immunoprecipitated complex confirmed that it contained the FLAG-tagged KMT2A/MLL1 and also Msk1, but not NFκB, a known interaction partner of Msk1  readily detected in the lysate (Fig. 1c). It seems that the KMT2A/MLL1–Msk1 interaction is specific, stable and present in a range of mouse and human cell types.
KMT2A and Msk1 knockdowns trigger similar expression changes in KMT2A target genes
We analysed the possible functional impact of the KMT2A/MLL1–Msk1 interaction by using a knockdown approach in MEFs. These cells express HoxA genes, known KMT2A/MLL1 targets . Transient siRNA-mediated knockdown conditions were established for KMT2A/MLL1, resulting in a >40% reduction in the protein (Fig. 2a) that was well tolerated by the cells. The knockdown was specific, with no impact on the transcripts of other mouse KMT genes (Fig. 2b). For Msk1, we established well-tolerated knockdown conditions that reduced Msk1 protein abundance by >90% (Fig. 2c), whilst leaving protein and transcript levels of its close paralogue Msk2 unaffected (Fig. 2c, d).
We used expression analysis on high-density microarrays to identify genes whose expression was sensitive to knockdown of KMT2A/MLL1 or Msk1. Using a fold change cut-off of 2 and false discovery rate <10%, 3912 different genes changed expression in response to either KMT2A/MLL1 or Msk1 knockdown. Undirected cluster analysis (SOTA) separated the responding genes into five groups, as shown in Fig. 2e. The up- and down-regulation of selected genes was validated by RT-qPCR (Additional file 2).
Strikingly, all 758 genes that were significantly down-regulated in response to the loss of KMT2A/MLL1 [cluster 3 genes] were also down-regulated in response to the loss of Msk1 (Fig. 2f, upper panel). Thus, all genes whose ongoing transcriptional activity is dependent on KMT2A/MLL1 (i.e. that are down-regulated by KMT2A KD) are also dependent on Msk1. The overlap of responding genes was much greater than expected by chance (Chi-squared test with Yates correction, two-tailed p value <0.0001), indicating a functional relationship between the two enzymes. This 100% overlap is not attributable to down-regulation of Msk1 in KMT2A/MLL1 KD cells or vice versa. Expression of KMT2A/MLL1 in Msk1 knockdown cells was not significantly altered, whereas the expression of Msk1 showed a modest up-regulation in response to the loss of KMT2A/MLL1 (Additional file 3).
Likewise, of the genes up-regulated in response to KMT2A/MLL1 KD, a majority (448, 62%) were also up-regulated in response to Msk1 KD (Fig. 2f, lower panel). However, these overlapping, up-regulated genes represent only a minority (19%) of the 2331 genes up-regulated in total by Msk1 KD (i.e. genes whose ongoing activity is repressed by Msk1). It seems that Msk1 alone, acting either directly or indirectly through phosphorylation of regulators such as NFkB or CREB [39, 41], is frequently involved in transcriptional repression.
Ontology analysis of significant genes was consistent with KMT2A/MLL1 and Msk1 activating a wide range of gene targets (Additional file 4). Genes down-regulated by KMT2A/MLL1 and Msk1 KD were modestly (1.5–1.9-fold), though significantly enriched in general terms such as glycoprotein and extracellular region activity. In contrast, genes down-regulated by KD of Msk1 alone were enriched selectively in cell cycle terms (130 out of 543 genes), with high enrichments (3.5–9.8-fold). The gene set whose activity depends on Msk1 alone differs fundamentally from that requiring both Msk1 and KMT2A/MLL1. These very different gene sets may account for the different degrees of knockdown tolerated by the cells, almost complete for Msk1 and ~40% for KMT2A/MLL1 (Fig. 2c).
KMT2A/MLL1 brings Msk1 to HoxA target genes to regulate their expression
We examined the effects of KMT2A/MLL1 and Msk1 knockdown on expression of genes within the HoxA cluster, important and well-described KMT2A/MLL1 target genes. Knockdown of KMT2A/MLL1 resulted in significant down-regulation of multiple HoxA genes, particularly those nearer the centre of the cluster (Fig. 3a). Msk1 knockdown generated a pattern of HoxA gene expression remarkably similar to that in KMT2A/MLL1 knockdown cells, with genes nearer the centre of the cluster, HoxA3–6 and HoxA10, showing the greatest down-regulation (Fig. 3b).
In order to understand better the functional relationship between KMT2A/MLL1 and Msk1, we examined their interaction at the HoxA4 and HoxA5 loci, near the centre of the cluster and amongst the most severely down-regulated genes in both knockdowns. Using ChIP-PCR with antibodies to phosphorylated Msk1, using three primer pairs spanning the gene, we found that Msk1 is normally enriched over the HoxA4 TSS (Fig. 3c, upper left panel). This enrichment is lost when KMT2A/MLL1 is knocked down (Fig. 3c, upper left panel), suggesting that Msk1 must interact with the MLL1 complex to be recruited to these sites. In contrast, whilst a peak of KMT2A/MLL1 was detected over the HoxA4 TSS, it did not change significantly upon Msk1 knockdown (Fig. 3c, upper right panel), indicating that Msk1 is not essential for the recruitment of the MLL1 complex. Exactly the same response to KMT2A/MLL1 and Msk1 KD was detected across the HoxA5 locus (Fig. 3c, lower panels). It is interesting to note that the peaks of KMT2A/MLL1 and Msk1 are located at the TSS of HoxA5, but upstream for HoxA4; so, their co-location is not simple because they both are located at TSS (Fig. 3c).
To define how KMT2A/MLL1 and Msk1 interact to regulate H3 modification levels at MLL1 complex target loci, we characterised key histone modifications at HoxA4 and HoxA5 in the context of KMT2A/MLL1 and Msk1 knockdown (Fig. 4a). Both H3K4me3 and H3K9acS10ph were enriched specifically at the TSS (left and middle panels). H3K4me3 fell to baseline levels, both upon KMT2A/MLL1 knockdown as expected and also in Msk1 knockdown cells (Fig. 4a, left panels). Likewise, the TSS-associated mark H3K9acS10ph was reduced both in Msk1 knockdown cells as expected and in KMT2A/MLL1 knockdown cells (Fig. 4a, middle panels). In contrast, the abundance of the silencing modification H3K27me3, used as a control, did not change upon knockdown of either protein (Fig. 4a, right panels). These results show that the KMT2A/MLL1 complex is required for recruitment of Msk1 to the HoxA4 and HoxA5 loci, and consistent with the proposition that Msk1-catalysed H3S10 phosphorylation facilitates the KMT2A/MLL1 complexes methyltransferase activity at these TSSs. However, the peaks of H3K4me3 and H3K9acS10ph at the HoxA4 TSS (Fig. 4a) do not coincide with the peaks of KMT2A and Msk1 at the same locus (Fig. 3c). Thus, whilst KMT2A and Msk1 are clearly necessary for the deposition of these histone modifications, the amount of enzyme at particular locations is not the sole determinant of their levels.
The KMT2A/MLL1 is necessary for regulation of genes with key roles in differentiation and development [1, 2] [42–44]. It has multiple domains that mediate binding to both proteins and DNA, and allow assembly of the multi-protein complex that regulates gene expression. KMT2A/MLL1 has a SET domain that, within the complex, confers lysine methyltransferase activity specific for histone H3 lysine 4 (H3K4), and is essential for gene regulation [3, 19]. The various proteins that form, or associate with, the core MLL1 complex regulate both its ability to methylate H3K4 and its ability to locate and gain access to its target genes, but the mechanisms that underpin these processes are not yet understood [1, 7]. Here, we show that the kinase Msk1, part of the MAPK signalling pathway, physically interacts with the MLL1 complex, is essential for the regulation of KMT2A/MLL1 target genes and, at HoxA4 and HoxA5, that this is via regulation of H3K4 methylation.
Four lines of evidence demonstrate the physical association of Msk1 with the KMT2A/MLL1 complex. First, KMT2A/MLL1 and Msk1 co-immunoprecipitate from extracts of several different cell types. Second, the two proteins co-localise across HoxA genes (known targets). Third, knockdown of Msk1 diminishes methylation of H3K4 at MLL1 complex target sites. Likewise, KMT2A/MLL1 knockdown diminishes H3S10 phosphorylation at the same sites, suggesting that, in the absence of the KMT2A/MLL1 complex, Msk1 is not appropriately targeted. This was confirmed by showing that KMT2A/MLL1 knockdown prevented Msk1 recruitment to HoxA4 and HoxA5 genes, whilst Msk1 knockdown did not disrupt KMT2A/MLL1 binding. Finally, our finding that expression of 81% of KMT2A/MLL1-regulated genes are influenced by Msk1 knockdown, shows that the functional interaction between Msk1 and the KMT2A/MLL1 complex in vivo is very frequent. Because our knockdown experiments targeting Msk1 left its close homologue Msk2 unaffected, we conclude that the effects we observe are mediated by Msk1 alone.
Msk1 is known to phosphorylate histone H3 at serines 10 and 28, as well as key regulatory proteins such as cAMP response element binding (CREB)  and NFκB . All of these substrates can, potentially, alter gene expression. The choice of which substrate, or even which lysine, to phosphorylate is likely to be context dependent [45, 46]. The catalytic properties of Msk1, previous data and the present results suggest that the enzyme regulates the KMT2A/MLL1 complex in one or both of two general ways, namely by enhancing SET domain-catalysed H3K4 methylation and/or by allowing the complex to access condensed chromatin. We have shown previously that SET domain methyltransferase activity is enhanced by the acetylation and phosphorylation of the H3 substrate at lysines 9 and 14 and serine 10, respectively . The most effective catalytic improvement was provided by the fully modified substrate (H3K9acS10phK14ac) . We have now shown that recruitment of Msk1 to KMT2A/MLL1 responsive genes and consequent increases in H3S10 phosphorylation leads to increased H3K4 methylation and up-regulation of transcription. In light of these data, we propose that (in addition to its other activities) Msk1-mediated phosphorylation of H3S10 directly stimulates the ability of the KMT2A/MLL1 SET domain to methylate H3K4 in vivo. It may be that the recruitment of the lysine acetyltransferase CBP by the KMT2A/MLL1 complex  also contributes to enhancement of H3K4 methylation through enhanced acetylation of H3K9 and K14. This model is shown in Fig. 4b.
H3 serines 10 and 28 are both adjacent to lysine residues whose various modifications contribute to long- and short-term control of gene expression. H3K9 acetylation is associated with active gene promoters  whilst its methylation is associated with silent, condensed chromatin (including centric heterochromatin) . H3K9me-mediated chromatin condensation is brought about largely through selective binding of heterochromatin protein 1 (HP1) [49, 50]. Msk1 phosphorylation of H3S10 appears to act in this process as a ‘phospho-switch’ that can block HP1 binding to H3K9me3, and even displace pre-bound HP1 [51, 52], thereby preventing or reversing gene silencing. This has been characterised on the mouse mammary tumour virus (MMTV) promoter, where Msk1 recruitment, along with the progesterone receptor (PR) and the extracellular signal-regulated kinase (Erk), leads to phosphorylation of H3S10 at the promoter, displacement of HP1 and recruitment of RNA polymerase II and the activator protein Brg1 . We propose that Msk1 facilitates binding of the KMT2A/MLL1 complex to some target genes by H3S10 phosphorylation and consequent diminution of HP1-mediated chromatin condensation (Fig. 4b).
Methylation of H3K27 is necessary for gene silencing by the Polycomb repression complex PRC2  and is put in place, and bound, by a component of the complex, the methyltransferase Ezh2 . Genes can be protected against PRC2-mediated silencing by H3K27 acetylation , and by modification of other H3 lysines [46, 57–59], but phosphorylation of H3 at serine 28, catalysed by MSK1/2, is known to displace PRC2, presumably by disrupting binding to the adjacent, methylated residue [60, 61]. This is consistent with the increase in H3K28ph abundance observed at the promoter regions of genes that are transcriptionally activated when quiescent 3T3 cells are stimulated back into growth . H3S28 phosphorylation allows Polycomb-silenced genes to be reactivated without the need to demethylate H3K27 and may play a role in allowing the KMT2A/MLL1 complex to access such genes.
In light of these results, the association of Msk1 with the MLL1 complex confers the potential ability to reverse both HP1-mediated and Polycomb-mediated chromatin silencing, as a prelude to selective gene activation. Whatever the detailed mechanisms may prove to be, the close physical and functional association between the KMT2A/MLL1 complex and Msk1 indicates novel routes by which the complex can implement its role as a transcriptional activator and developmental regulator.
The histone kinase Msk1 physically associates in vivo with the KMT2A/MLL1 complex in human and mouse cells and is essential for the regulation of key developmental target genes. This represents a direct functional link between the MAPK cell signalling pathway and KMT2A-/MLL1-mediated gene regulation. At least, part of this regulatory role is accomplished through histone modification, specifically methylation of H3K4 and phosphorylation of H3S10 and possibly H3S28.
Human lymphoblastoid cells (LCLs) with a normal karyotype were generated by M. Rowe (Uni. Birmingham) and grown in RPMI 1640, 100 µg/ml streptomycin, 100 U/ml penicillin and 10% FBS (Foetal bovine serum, Invitrogen) at 37 °C, 5% CO2. Human embryonic kidney cells (HEKs) were cultured in DMEM, 100 µg/ml streptomycin, 100 U/ml penicillin and 10% FBS at 37 °C, 5% CO2. Mouse embryonic fibroblasts (MEFs) were generated in-house, via isolation from Balb/c wild-type mouse embryos between day 12 and 15 of gestation, and cultured in DMEM, 100 µg/ml streptomycin, 100 U/ml penicillin, 1/100 MEM non-essential amino acids, 1/1000 2-mercaptoethanol and 10% FBS at 37 °C, 5% CO2. Only cells between passage 2 and 4 were used. Drosophila SL2 (Schneider line-2) cells were cultured in Schneider’s medium supplemented with 8% FBS and 1/100 penicillin/streptomycin, and incubated at 26 °C.
RNA extraction and RT-qPCR
RNA was isolated using RNeasy Minikit (Quaigen), and reverse transcription/cDNA amplification performed in one step using QuantiTech™ Sybr® Green Mix (Qiagen), according to the manufacturer’s instructions. Reactions used commercial primers (Qiagen: Msk1 QT00141554, actin QT01336772, HoxA1 QT00248322, HoxA2 QT0062812, HoxA3 QT00138264, HoxA4 QT00174986, HoxA5 QT00098819, HoxA6 QT00140742, HoxA7 QT00168707, HoxA9 QT00108885, HoxA10 QT00240212, HoxA11 QT00250404, HoxA13 QT0027642, MLL1 QT00240954, MLL2 QT01075620, MLL3 QT00310527, MLL5 QT00279426), with three analyses performed for each sample. RT-qPCR was performed on a 7900 HT machine (Applied Biosystems).
Isolation of proteins and detection
Cells were washed and incubated in lysis buffer [20 mM Tris–HCl pH 7.4, 100 mM EDTA, 10 mM NaCl, 1% Triton X-100, 1 mM β-glycerophosphate, 1 mM EGTA, 5 mM sodium pyrophosphate, ‘Complete’ protease inhibitor (Roche)] for 30 min on ice. Insoluble material was removed by centrifugation and protein concentration determined by Coomassie Plus protein assay (Thermo Scientific). Proteins were separated by gel electrophoresis and stained with silver nitrate or blotted for western analysis. Primary antibody binding was detected by fluorescently tagged secondary antibodies (Licor) and gel loading normalised using anti-actin or anti-Pol II antibodies (Abcam).
Antibody for H3K4me3 (R612) was generated in-house [63, 64]. Other antibodies were obtained commercially: H3K9acS10p [Abcam ab12181]; H3K27me3 [Millipore 07-449]; phosphorylated Msk1, this antibody is specific for Msk1 phosphorylated at S360, one of the last residues to become phosphorylated and detects active Msk1 [Abcam ab81294]; KMT2A [Millipore 05-764]; Msk2 [R&D systems MAB2310]; actin [Abcam ab1801]; RNA Pol II [Abcam ab5408]; NFκB [Abcam ab7970-1]; FLAG [Sigma F1804].
Chromatin immunoprecipitation (ChIP)
Immunoprecipitation of micrococcal nuclease-digested, native chromatin coupled to quantitative PCR (qPCR) was used to determine levels of histone modification at selected genomic regions, as previously described [65, 66].
Immunoprecipitation of formaldehyde cross-linked chromatin (X-ChIP) in MEFs was carried out essentially as described [67, 68]. Briefly, MEFs were cross-linked with 1% para-formaldehyde in medium for 10 min at RT. The reaction was quenched by the addition of 200 mM glycine before washing the cells twice with ice-cold PBS. Cells were resuspended in lysis buffer [1% SDS, 10 mM EDTA, 50 mM Tris–HCl, pH 8, complete inhibitor cocktail(Roche)] to a concentration of 105 cells in 500 μl and sonicated at high power with 30 s ON/OFF for 7 cycles (Bioruptor, Diagenode). The chromatin was diluted 1:2 with dilution buffer [1% Triton X-100, 2 mM EDTA, 20 mM Tris–HCl, pH 8, 150 mM NaCl, complete inhibitor cocktail (Roche)]. Protein G Dynabeads (Thermo Fischer) were prepared resuspended in citrate–phosphate buffer (0.1 M citric acid, 0.1 M Na2HPO4) with 0.5% BSA. Beads were coated with antibodies for 2 h at 4 °C. The beads were washed twice, added to the chromatin solution and incubated for 4 h at 4 °C. The beads were washed twice with low-salt buffer (1% Triton X-100, 0.1% SDS, 2 mM EDTA, 150 mM NaCl, 20 mM Tris–HCl, pH 8) and twice with high-salt buffer (1% Triton X-100, 0.1% SDS, 2 mM EDTA, 100 mM NaCl, 20 mM Tris–HCl, pH 8). The bound material was eluted with Elution buffer (100 mM NaHCO3, 1% SDS decross-linked, and the DNA extracted with QIAquick PCR purification kit (QIAGEN). Primer pairs used for analysing ChIP DNA by qPCR are listed in Additional file 5.
Co-immunoprecipitation experiments were carried out as described before, using 1 × 108 cells per precipitation and in the presence of 50 µg/ml ethidium bromide, in order to release chromatin bound proteins from DNA [69–72]. Cells were lysed in NP40 buffer [1% NP40, 10% glycerol, 50 mM Tris pH 7.5, 0.1% sodium azide, 150 mM NaCl, complete proteinase inhibitor (Roche)] for 20 min on ice before sonication (high power, 10 s, Bioruptor Diagenode). The lysate was cleared by centrifugation (10 min, 17000g, 4 °C) and pre-cleaned by adding protein A Sepharose beads for 30 min at 4 °C. The cleaned lysate was incubated overnight with 15 µg of antibody at 4 °C on a rotating wheel and then with 40 µl beads for 3 h at RT. The beads were washed three times with high-salt buffer (1% NP40, 10% glycerol, 50 mM Tris pH 7.5, 0.1% sodium azide, 200 mM NaCl). The beads were analysed on a SDS gel and western analysis, together with a no-antibody control and 10% input samples.
Overexpression of FLAG-MLL1 and FLAG precipitation
HEK293 cells were split the day before transfection and 4 × 106 cells seeded into 10 cm plates in complete medium. The next day, a mix of 15 μg vector DNA (pMSCV-FLAG-MLL1, R. Slany, University Erlangen), 1000 μl OptiMEM (Gibco) and 60 μl of polyethyleneimine (1 mg/ml, Polyscience Inc.) per plate was incubated for 15 min at RT prior to addition to the plates. The cells were cultured at 37 °C, 5% CO2 for 3 days and harvested, washed with TBS and resuspended in lysis buffer (1× TBS, 1 mM EDTA, 1% TritonX-100). Cells were incubated on ice for 30 min prior to centrifuging (15 min, 13,000 rpm, 4 °C), addition of 50 μl anti-FLAG M2 magnetic beads (Sigma) and incubation for 2 h at RT with rotation. Beads were washed with TBS and FLAG-MLL1 was eluted using 5 μg of 3× FLAG peptide (Sigma) and diluted in 500 μl TBS. The beads were incubated twice with 250 μl elution for 30 min, 4 °C with rotation and the supernatants pooled.
shRNA vectors were purchased from Origene (Msk1: TR504795; MLL1: TR517798) and transfected into MEFs by electroporation (GenePulser XCell, Bio-Rad). MEFs were brought into a single cell suspension by trypsination and washed with ice-cold PBS. 4 × 106 cells per transfection were resuspended in 400 μl electroporation buffer (10 mM Hepes, pH 7.5, 135 mM KCl, 2 mM MgCl2, 5 mM EGTA, 25% FBS) and transferred to a 4 mm electroporation cuvette (Bio-Rad). 20 μg of vector DNA was added to the cells immediately before transfection and incubated on ice for 1 min. Cells were transfected with one pulse (300 V, 600 μF, 1000 Ω) and allowed to recover (RT, 1 min), before addition of 10 ml pre-warmed MEF medium and incubation at 37 °C, 5% CO2 for 6 h. Non-transfected cells were removed by puromycin treatment (10 μg/ml, 14 h). After 20 h, cells were harvested. The KD efficiency is determined by quantitation of Wester blots using the corresponding loading controls for normalisation.
Gene expression arrays
Expression analysis was performed on MEFs transfected with knockdown shRNA vectors, using NimbleGen arrays (100718_MM9_EXP_HX12) containing 12 individual 135 k arrays with 3 probes per sequence for 42,576 sequences. 10 μg of RNA per array was isolated (RNeasy, Qiagen) and reverse transcribed using the cDNA synthesis system (NimbleGen). cDNA samples were labelled with the NimbleGen One-Colour DNA labelling kit and subsequently hybridised on the array, washed and scanned with the MS 200 Microarray Scanner (NimbleGen). Four replicate transfections were carried out for each knockdown and control but one control and one MSK1 KD replicate were eliminated as outliers. Data were extracted in DEVA (Nimblegen), processed with R (background correction and normalisation) and analysed with MeV (clustering, statistical analysis and visualisation). Statistical significance of co-regulated genes was analysed by 2 × 2 contingency tables and Chi-squared analysis with Yates correction (GraphPad, www.graphpad.com). Microarray data have been deposited in GEO (www.ncbi.nlm.nih.gov/geo), accession number GSE89141.
absent, small or homeotic 2-like
nuclear mitogen- and stress-activated protein kinase
nuclear factor κB
retinoblastoma-binding protein 5
- SET domain:
suppressor of variegation, enhancer of zeste, trithorax domain
self-organising tree algorithm
transcription start site
WD-40 repeat 5
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MW devised and carried out experiments, prepared figures and wrote the paper; MB devised and carried out experiments; JAH analysed data and wrote the paper; NT analysed data; RS supplied reagents and advised on interpretation of data; BMT wrote the paper and advised on interpretation of data; and KPN devised experiments, interpreted data and wrote the paper. All authors read and approved the final manuscript.
The authors thank Dr. F. Tavner for establishing KMT2a knockdown procedures. Funding was supported by Cancer Research UK (Grant No. C1015/A13794).
The authors declare that they have no competing interests.
This work was supported by project grants from Cancer Research UK and a studentship (MW) from the BBSRC.
Additional file 1. Co-immunoprecipitation of KMT2A and Msk1. The complete membranes from the Western blot images shown in Fig. 1a, b.
Additional file 2. Validation of microarray data. Primer pairs for randomly selected genes that were up- (Hemt1/Hsph1/Ifnar2/Tet3) or down-regulated 9Il1b/Ly9/Ttk/Kif20b) according to the microarray analysis were designed. The genes were tested with RT-qPCR on KMT2A/MLL1 knockdown or Msk1 knockdown RNA (n = 3).
Additional file 3. Knockdown of KMT2A/MLL1 does not diminish Msk1, nor does Msk1 KD diminish KMT2A. Changes in KMT2A/MLL1 and Msk1 transcript abundance in KMT2A/MLL1 and Msk1 knockdown cells. Transcript levels are presented as fold change and normalised to the mock-transfected controls (n = 3/4, T-test p < 0.05*, p < 0.01**, p < 0.001***).
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Wiersma, M., Bussiere, M., Halsall, J.A. et al. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes. Epigenetics & Chromatin 9, 52 (2016). https://doi.org/10.1186/s13072-016-0103-3
- MLL1 complex
- Histone modification
- Signal transduction
- Gene regulation