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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes

Fig. 3

HoxA gene expression and the distribution of KMT2A/MLL1 and Msk1 change upon KMT2A/MLL1 or Msk1 knockdown. a RT-qPCR expression analysis of HoxA genes in KMT2A knockdown cells, compared to mock-transfected control cells normalised to 1.0 (n = 3, T test, *p < 0.05; **p < 0.01; ***p < 0.001). b RT-qPCR expression analysis of changes in expression of HoxA genes in Msk1 knockdown cells (n = 3, T test, *p < 0.05; **p < 0.01; ***p < 0.001). c Effect of KMT2A/MLL1 knockdown on Msk1 binding (upper panels) or Msk1 knockdown on KMT2A binding (lower panels) across the HoxA4 gene (left hand panels) and HoxA5 gene (right hand panels). Binding was normalised to mock-transfected cells (n = 3, T test, *p < 0.05; **p < 0.01; ***p < 0.001). Gene maps for HoxA4 and HoxA5 show exons (boxes), TSS (upper arrow) and the locations of primers 1–3 (lower bars)

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Epigenetics & Chromatin

ISSN: 1756-8935