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Chromatin structure and DNA damage repair
Epigenetics & Chromatin volume 1, Article number: 9 (2008)
The integrity of the genome is continuously challenged by both endogenous and exogenous DNA damaging agents. These damaging agents can induce a wide variety of lesions in the DNA, such as double strand breaks, single strand breaks, oxidative lesions and pyrimidine dimers. The cell has evolved intricate DNA damage response mechanisms to counteract the genotoxic effects of these lesions. The two main features of the DNA damage response mechanisms are cell-cycle checkpoint activation and, at the heart of the response, DNA repair. For both damage signalling and repair, chromatin remodelling is most likely a prerequisite. Here, we discuss current knowledge on chromatin remodelling with respect to the cellular response to DNA damage, with emphasis on the response to lesions resolved by nucleotide excision repair. We will discuss the role of histone modifications as well as their displacement or exchange in nucleotide excision repair and make a comparison with their requirement in transcription and double strand break repair.
Proper functioning of all living organisms depends on faithful maintenance of genomic information. Although it is generally believed that information stored is relatively safe and stable, the integrity of DNA is continuously challenged by numerous genotoxic agents and environmental stress. Essential cellular functions such as oxidative respiration and lipid peroxidation create reactive oxygen species that can damage DNA. In addition, spontaneous hydrolysis of nucleotides induces non-instructive abasic sites. Finally, environmental physical and chemical agents, such as ultraviolet (UV) and ionising radiation, as well as numerous genotoxic chemicals present in food or combustion products in the air, induce a wide variety of DNA lesions. It has been estimated that in an average mammalian cell ten to a hundred thousand DNA lesions are introduced each day .
The consequences of DNA damage are diverse and adverse. Acute cellular effects arise from impeded gene transcription and DNA replication, causing cellular malfunctioning, irreversible cell cycle arrest (senescence) or cell death (apoptosis) which are important factors in (premature) aging [2, 3]. DNA lesions interfere with proper chromosome segregation during cell division resulting in chromosome aberrations. In addition, replication errors due to DNA damage may introduce irreversible mutations. Chromosomal aberrations as well as mutations in coding genes may lead to carcinogenesis .
To counteract the severe biological consequences of DNA lesions an intricate network of genome surveillance mechanisms or DNA damage response (DDR) processes has evolved. The heart of this defence system is formed by complementary DNA repair systems that cover most of the genetic insults. In addition to the direct removal of lesions, DNA injuries trigger a signalling cascade that results in a slowdown of cell cycle progression, providing cells more time to repair DNA damage prior to replication or cell division.
The template for the DDR, damaged DNA, is packed into chromatin and it is expected that, analogous to other chromatin-associated processes such as replication and transcription [4, 5], chromatin structure influences the DDR and vice versa. Recently, an array of different types of chromatin structural modulations has been reported in relation to the DDR. In this review we summarize and discuss current knowledge on chromatin remodelling, with emphasis on one specific DNA repair process, nucleotide excision repair (NER). For recent reviews on the connection between chromatin remodelling and repair pathways other than NER, see [6–14].
The many different types of DNA lesions cannot be repaired by a single repair system. Instead, a number of specific repair processes have evolved that each remove a subset of lesions [15, 16]. At least four major damage repair pathways operate in mammals: NER, base excision repair (BER), homologous recombination (HR) and end joining (EJ). NER deals with the wide class of single-strand lesions that destabilize the double helix, potentially obstructing transcription and replication. Small types of chemical alterations in the bases and single-strand DNA breaks are targeted by BER. Lesions for NER and BER affect only one of the strands of the double helix. In both processes the injury is excised and the resulting gap is filled by DNA synthesis using the intact complementary strand as a template, enabling error-free DNA repair. To properly heal the more problematic double-strand breaks (DSBs) two major pathways have developed. In mammals HR appears to be the predominant mode of DSB repair in S and G2, when an intact second copy of the sequence (sister chromatid) is available. The more error-prone non-homologous EJ operates mainly in G1 phase, but can also work on DSBs in S phase.
NER is a versatile repair pathway able to remove many different types of single-strand lesions including the major UV-induced DNA photoproducts: cyclobutane pyrimidine dimers and 6-4 photoproducts, as well as large or bulky chemical adducts . In actively transcribed genes, such lesions cause stalling of RNA polymerase II, which in turn recruits downstream NER proteins, a pathway termed transcription-coupled NER (TC-NER) . In regions of the genome that are not actively transcribed, helix-distorting lesions are detected by the collective action of the UV-damaged DNA-binding protein (UV-DDB) complex and the xeroderma pigmentosum group C (XPC)-containing complex, initiating the global genome NER (GG-NER) sub-pathway . Both UV-DDB and XPC complexes are able to bind to a surprisingly broad range of lesions that create short stretches of unpaired bases. The substrate versatility of the XPC complex is achieved by binding to the unpaired bases in the non-damaged strand opposite the lesion [19, 20]. After recognition of the damage by either stalled RNA polymerase II or the XPC complex, repair complexes are assembled from freely diffusing NER factors (Figure 1, showing only the GG-NER pathway). The two helicases (XPB and XPD as part of the multi-functional, multi-subunit repair/transcription factor TFIIH), further unwind the helix after which two single-strand DNA-binding proteins (XPA and RPA) stabilize the unwound structure and properly orient the two structure-specific endonucleases (XPF-ERCC1 and XPG) that incise at some distance at either site of the lesion. The resulting 25 to 30 nucleotide single-strand gap is filled by the DNA replication machinery and finally sealed by one of the two DNA ligases (Ligase1 and XRCC1-Lig3) [16, 21–24].
Cell-cycle checkpoint activation
To allow a cell to repair DNA lesions before it replicates over lesions that reduce the fidelity of polymerases (causing mutations) or passes through mitosis and propagates potentially harmful mutations to the daughter cells, DNA damage checkpoints temporarily block the cell cycle in G1, S or G2 phases in response to genotoxic stress. The three mammalian phosphoinositide 3-kinase-like kinases (PIKKs) ATM, ATR and DNA-PKcs have a central role in the activation of DNA damage checkpoints. These kinases induce a cascade of phosphorylations on a large number of different substrates, via mediators and transducers to effector molecules, such as the checkpoint kinases Chk1 and Chk2 [25–28]. Other targets include the histone H2A variant H2AX, checkpoint mediator protein 53BP1, DSB recognition factor NBS1 and many others [29, 30]. ATM, ATR and DNA-PKcs are not only required for activating cell-cycle checkpoints but they also phosphorylate many substrates involved in other aspects of the DDR.
Although most types of DNA lesion have the ability to trigger cell-cycle checkpoint activation, damage signalling has been mainly studied in relation to DSBs. DNA DSBs induced by ionizing radiation generally cause large-scale chromatin rearrangements, initiated by ATM-dependent phosphorylation of H2AX (see below) . Phosphorylated H2AX (γH2AX) triggers the accumulation of a multitude of different DSB repair and DNA damage signalling molecules, thereby concentrating repair proteins in small discrete nuclear foci termed ionizing radiation-induced foci (IRIF). Although their nature and function has been a topic of debate, it is believed that they at least play a role in the signalling pathway. Furthermore, it has been proposed that a function of increased local concentrations of proteins involved in the DDR will both stimulate repair and serve as an amplification of checkpoint signals [32, 33]. As even a single endonuclease-induced DSB can induce chromosomal instabilities , amplification of checkpoint signals is most likely required to block the cell cycle until the DSB is repaired.
Proteins involved in NER typically bind to areas of DNA damage resulting in temporary immobilization of the proteins, but rather than accumulating in foci, they retain a homogenous distribution in G1 and G2 [35, 36]. This also suggests that signal amplification by increased protein concentrations through foci formation, whether for activation of a cell-cycle checkpoint or for more efficient repair, does not play a role in NER. In addition, there is no evidence that NER-inducing lesions directly activate one of the PIKKs. However, during S-phase IRIF-like foci are formed when NER lesions generate stalled DNA replication forks, which create single-stranded stretches that are quickly covered by RPA/ATRIP and finally activate ATR, triggering replication-stress signalling [37, 38]. Recently however, evidence has been provided that NER intermediates (short single-strand gaps, resulting from excised lesions) also activate ATR [39, 40] outside S phase. It is currently unknown whether NER-induced ATR-signalling requires amplification in another way than by formation of foci as seen in DSB repair.
In transcription and replication, changes in the chromatin structure are required in order to allow binding of the factors involved [4, 5]. There is increasing evidence that this is also the case for DNA repair. Chromatin remodelling to alter the accessibility of proteins to DNA occurs by two mechanisms: covalent histone modifications by means of post-translational modifications (PTMs) and displacement of histones or entire nucleosomes, either by sliding along the DNA or by removal. In this review we will discuss recent literature addressing questions of if and how the DDR, and more particularly NER, requires modifications of chromatin structure and whether sequence-specific epigenetic marks are restored after repair. Figure 2 presents a model of known and speculated NER-associated chromatin modifications and the factors that are involved. In addition, in Tables 1 and 2 NER-related chromatin remodelling events are summarized and compared with these events during transcription.
Covalent histone modifications or epigenetic changes are important regulatory elements for many biological processes. They function by influencing chromatin contacts through structural histone changes or influencing electrostatic interactions, and by recruiting non-histone proteins to chromatin . Some covalent histone modifications that are involved in transcription are also associated with repair. On the other hand, modifications such as phosphorylation of H2AX appear to be unique to the DDR. In the following sections we will focus on the four epigenetic marks that are implicated in the DDR: phosphorylation, acetylation, methylation and ubiquitination. For a comprehensive review comparing covalent histone modifications during the different repair processes, see .
The hallmark DDR-related epigenetic change is phosphorylation of H2AX. Upon DSB induction by ionizing radiation, histone H2AX in the vicinity of DSBs is phosphorylated by ATM and DNA-PKcs at Serine 139. H2AX is phosphorylated over surprisingly long stretches of DNA of up to 2 × 106 bp around the break [8, 31, 42, 43], creating a robust chromatin mark. In DSB repair, γH2AX is important for the formation of IRIF. In the absence of γH2AX, repair factors such as NBS1 (part of the homologous recombination-essential complex MRN) and Brca1 as well as checkpoint proteins MDC1 and 53BP1 fail to accumulate in IRIF, although they are still recruited to DNA damage . Despite the dramatic chromatin changes and highly concentrated repair factors induced by γH2AX, its absence only mildly affects DSB repair . This indicates that γH2AX-induced concentration of repair factors at sites of DNA damage does not play a crucial role in DNA repair. However, the formation of these IRIF through phosphorylation of H2AX is important for the activation of cell-cycle checkpoints in response to low doses of ionizing radiation , whereas γH2AX is dispensable for checkpoint activation at higher doses .
H2AX is also phosphorylated at residue S139 in response to UV irradiation [39, 45–47], as well as its equivalent serine (S129 of H2A) in S. cerevisiae . However, a S129A mutant strain was only slightly UV sensitive. Rather, it appeared that three different serine residues, S2, S18 and S122 play important roles in survival under UV. The serine 122 residue is involved in a general response to DNA damage, as it is required for HR, non-homologous EJ and NER. Surprisingly, this residue becomes dephosphorylated rather than phosphorylated upon UV irradiation, whereas other damage-inducing agents cause an increase of phosphorylation of S122 . Whereas the DDR involves differential phosphorylation of multiple residues on both termini of H2A in yeast, no evidence is currently available that residues other than S139 of mammalian H2AX and H2A are implicated in the DDR.
Two histone residues of H3, serine 10 and threonine 11, appear to be a target of differential phosphorylation during NER [49, 50]. Phosphorylation of both these residues is associated with transcription activation [51, 52]. Like H2AS122 in yeast, H3S10 and H3T11 in mouse are dephosphorylated by UV irradiation and rephosphorylated after repair of the damage [49, 50]. The function of this H3 modification in the DDR is not yet known. It is however tempting to speculate that the non-histone chromosomal protein HMGN1 plays a role, since HMGN1 inhibits the phosphorylation of H3S10 [53, 54], enhances repair of UV lesions [53, 54] and was shown to be recruited to TC-NER complexes . A possible scenario for the role of HMGN1 in differential H3 phosphorylation within the DDR could be that HMGN1 is responsible for the dephosphorylation of H3S10. Hypophosphorylation of H3 at S10 and T11 is associated with transcription repression, and this might be one of the different mechanisms cells employ to inhibit transcription at UV-damaged areas. Dephosphorylation of S10 and T11 of H3 might also facilitate the acetylation of H3K9 (see below), the residue directly next to S10, by removing steric hindrance or neutralizing the negatively charged environment for an H3K9 acetyl transferase.
Another abundant histone PTM is differential acetylation, mainly associated with transcription activation. Different lysines in both histones H3 and H4 are targets for this modification, which neutralizes the basic charge of the lysine, thereby potentially altering the interaction between adjacent histones and between histones and DNA . It was shown 20 years ago that histones become hyperacetylated in response to UV irradiation and that DNA repair is more efficient in hyperacetylated nucleosomes [55, 56]. This suggests that changes in chromatin structure induced by acetylation make DNA more accessible not only for transcription factors but also for DNA repair activities.
Two histone acetyl transferases (HATs), Gcn5 and p300, responsible for the acetylation of multiple lysine residues within all four core histones, are implicated in the UV-induced DDR. In yeast, Gcn5 hyperacetylates H3 (at K9 and K14) at the repressed MFA2 promoter upon UV irradiation . This acetylation is accompanied by increased accessibility of the DNA template (as tested by activity of restriction enzymes at the promoter), suggesting that the function for this modification is to allow access of proteins to the damaged DNA. Histone H3 acetylation by Gcn5 is implicated in regulating gene expression of ~5% of the yeast genome, including at MFA2. Another promoter, RPB2, which does not require Gcn5 for histone acetylation, also does not require Gcn5 for more efficient damage removal . In mammalian cells, there is also evidence for the involvement of Gcn5 and the acetylation of H3 and H4 in the DDR. Gcn5 is recruited to DNA damage as part of a large complex, TFTC, which also includes SAP130, a splicing factor with sequence homology to DDB1, which is one of the subunits of the UV-DDB complex. Gcn5 is also part of another complex, STAGA, which interacts with both SAP130 and DDB1 in HeLa . In yeast, the Rad16/Rad7 complex is implicated in UV-induced histone acetylation . This protein complex is essential for yeast GG-NER [61, 62]. Although GG-NER is conserved to mammals, surprisingly no sequence homologues for Rad16 or Rad7 have been found in mammalian cells. However, it has been suggested that the UV-DDB complex (containing DDB1 and the GG-NER-specific DDB2) might be a functional homologue of Rad16/Rad7 . Besides the involvement of both these complexes in histone acetylation upon UV irradiation, there is more evidence pointing towards at least a partial functional homology between these complexes. For example, both are also involved in early steps of GG-NER and the ubiquitination of the NER recognition factor XPC/Rad4 [64, 65].
Gcn5 regulates a subset of genes, whereas p300 HAT is a more global regulator of transcription . Multiple proteins have been implicated in targeting p300 to UV damage, including Ing1B , DDB1 , PCNA , CSB  and p53 . This suggests that besides Gcn5, p300 also has a function in acetylating histones before, during or after NER, although no direct evidence has so far been presented that p300 activity is required for efficient NER.
A third abundant epigenetic histone mark is the differential methylation of lysine and arginine residues. Histones can be either mono-, di- or trimethylated and can either be a marker for transcriptionally active or inactive chromatin, depending on the type of methylation and the residue involved. Arginine methylation is less well studied than lysine methylation and no connection of this modification with a DDR has been found so far. Methylation of lysines H3K4, H3K36 and H3K79 is associated with transcription activation, while methylation of H3K9 and H3K27 and H4K20 is connected to transcription repression . In response to UV irradiation, two apparently antagonizing histone methylations, H3K79me and H4K20me, have been identified.
Studies in S. cerevisiae have shown that H3K79 methylation by Dot1p is required for efficient UV-damage response. Disruption of H3K79 methylation, by dot1 or K79E mutations, results in hypersensitivity to UV and intra-S phase checkpoint deficiency [71, 72]. In a similar study, H4K20 and its methyltransferase Set9 were mutated, resulting in UV sensitivity and checkpoint deficiency in fission yeast . Both H3K79 and H4K20 methylation are involved in checkpoint signalling after DSB induction by recruiting 53BP1/CRB2 to IRIF [73–77]. In addition to DSB, there are indications that 53BP1 is also activated by UV irradiation [29, 78–80]. This indicates that histone methylation might have the same checkpoint activation function after UV damage induction as it has in response to DSBs.
The largest PTM of histones is by conjugation of ubiquitin or ubiquitin-like moieties to lysine residues. Ubiquitin is a small 8.5 kDa peptide that can either target the conjugated protein to proteasomal degradation or serve as a modifier for protein function [81, 82]. Modification of proteins by ubiquitin usually occurs via a three-step enzymatic reaction, involving ubiquitin-activating, -conjugating and -ligating (respectively E1, E2 and E3) enzymes that conjugate either one (monoubiquitination) or multiple (polyubiquitination) ubiquitin moieties on target polypeptides, depending on the substrate-specific combination of used E2 and E3. All four core histones are targets for ubiquitination but the precise function for most histone ubiquitination activities remains obscure. During transcription, ubiquitination of H2A is generally associated with gene silencing whereas ubiquitination of H2B has been related to both gene activation and silencing [83–86]. Ubiquitination of H3 and H4 is less abundant and as yet no functional consequence has been assigned to this modification.
In yeast, histone H2B ubiquitination at lysine 123 by the Rad6/Bre1 E2/E3 complex is required in the response to several DNA damage sources, including UV [72, 87]. Absence of this modification (either by mutating Rad6 or H2B (K123R)), affects activation of the checkpoint kinase Rad53 and appears connected with exposure of lysine 79-methylated H3 and its subsequent activation of another checkpoint protein Rad9 [71, 72]. No evidence has yet been presented that ubiquitination of H2B is increased after DNA damage induction. In mammalian cells, H2A rather than H2B appears to be the main target of ubiquitination in response to UV irradiation . Remarkably, this modification is not required for NER, but rather occurs as a consequence of functional NER, as several defined NER mutants did not result in UV-induced H2A ubiquitination. Damage-induced H2A ubiquitination has been further shown to depend on ATR, which suggests that it has a function in cell cycle signalling.
UV irradiation not only causes an increase of H2A ubiquitination, but also induces a temporary H3 and H4 ubiquitination . A complex containing UV-DDB and CUL4A was required for this ubiquitination. H3 and H4 ubiquitination occurs early in the DDR, in contrast to H2A ubiquitination. Together with the notion that ubiquitinated H3 and H4 reduce nucleosomal stability, it has been suggested that by this ubiquitination the UV-DDB complex creates a chromatin environment that facilitates the assembly of the NER complex on damaged DNA .
Besides UV-induced H2A ubiquitination, ubiquitination of H2A and H2AX in response to DSB induction has also been recently observed [91–93]. This epigenetic change is dependent on the ubiquitin-conjugating (E2) enzyme Ubc13 and the E3-ligase RING finger-containing protein RNF8 [91–93]. The ubiquitination was shown to depend on H2AX phosphorylation and the subsequent recruitment and activation of MDC1, to which RNF8 binds. Ubiquitinated targets (H2A and H2AX) are crucial for the formation of IRIF and assembly of downstream repair and checkpoint factors (RAP80, BRCA1 and 53BP1). The dynamic equilibrium of differential H2A ubiquitination was shown to be important for genome stability as a mutant form of the H2A-specific deubiquitination (or DUB) enzyme USP3 causes delay of S phase progression and activation of checkpoints .
Histone displacement and exchange
ATP-dependent chromatin remodelling
Besides covalent histone modifications, another important mechanism that changes chromatin structure is accomplished by a series of ATP-dependent chromatin remodelling complexes. ATP-dependent chromatin remodelling involves displacement of histones, either by completely removing or replacing them, or by sliding whole nucleosomes along the DNA strand. Like covalent histone modifications, this activity can control the recruitment of DNA-interacting proteins to chromatin and it is a versatile control mechanism in all nuclear processes .
Next to a clear function in transcription regulation, a number of ATP-dependent remodellers have been shown to play a role during DSB repair, including SWR1, RSC, INO80, Rad54 and SWI/SNF [96–99]. Of these, only SWI/SNF has also been associated with NER in in vitro experiments . All ATP-dependent chromatin remodelling complexes contain a motor subunit belonging to Snf2-like family of ATPases . This Snf2-like family includes a few known DNA repair proteins such as Rad16 and Rad5 in yeast (no mammalian orthologues have been identified) and Rad54 and Rad26 (RAD54 and CSB in mammals, respectively). Within an in vitro accessibility assay the TC-NER-specific CSB disarranged regularly spaced nucleosomes in an ATP-dependent manner . Furthermore CSB directly interacted with double-stranded DNA and histone tails, which were required for this activity, suggesting that CSB may act as a TC-NER chromatin remodeller. CSB was also found to stimulate transcription by its interaction with RNA polymerase II, providing yet another link between DNA repair and transcription . The yeast Rad16, which harbours a Snf2-like domain, is exclusively involved in GG-NER , although no evidence exists for a role in chromatin remodelling. Rather, it acts in complex with Rad7 and Abf1 to generate superhelical torsion in DNA, and its activity appears to be hindered by intact nucleosomes [62, 104].
Two ATP-dependent chromatin remodellers that have been shown to stimulate NER by their remodelling activity are ACF and SWI/SNF [100, 105]. ACF is known to have a function in replication, especially of heterochromatin regions . It also enhances binding of heterochromatin protein 1 (HP1) to transcriptionally inactive chromatin and it does not co-localize with RNA polymerase II at salivary gland polytene chromosomes in Drosophila. This suggests that ACF is associated with transcription repression rather than activation [107, 108]. In contrast, SWI/SNF is mainly associated with transcription activation [109, 110].
ACF consists of two subunits, Acf1 and ISWI, which move nucleosomes along the DNA, generating internucleosomal spaces of 50 to 60 base pairs, without removing histones . This nucleosomal sliding enhances NER activity, mainly in the linker regions between nucleosomes . It will be interesting to investigate whether the mammalian orthologue of ACF is also implicated in the DDR. In contrast, the yeast SWI/SNF complex rather enhances NER of lesions located in nucleosome core regions . This in vitro remodelling activity by SWI/SNF is dependent on the presence of NER factors XPC, XPA and RPA. In addition, two subunits of the yeast SWI/SNF complex, Snf5 and Snf6, co-purified with the NER factors Rad4 (the yeast homologue of XPC) and Rad23 . Furthermore, Snf5 and Snf6 were shown to enhance NER and rearrange chromatin at the silent HML locus after UV irradiation. Most likely, yeast SWI/SNF is recruited to DNA lesions by binding to the Rad4-Rad23 complex, which is an early event in NER.
Genome function depends for a large part on the accessibility of the DNA template. Above, several mechanisms are summarized that provide more plasticity to the dense chromatin structure. However, long-range transactions on the DNA helix require more than simply increasing accessibility. During transcription, elongation and replication, polymerases progress over long distances on DNA and extended nucleoprotein filaments (involving RPA and RAD51) are formed in homologous recombination, which involves large-scale nucleosomal rearrangements. Although it is likely that the discussed chromatin remodellers provide sufficient space to allow these elongations, displaced nucleosomes need to be repositioned after termination of these reactions. While some ATP-dependent chromatin remodellers are able to (re)deposit histones onto DNA, it is likely that for these more robust chromatin changes specialized activities exist to restore the chromatin structure. These specialized enzymes are referred to as histone chaperones. Histone chaperones deposit core histones onto DNA in an ATP-independent manner .
ASF1 is a histone chaperone that works together with either CAF1 or HIRA to deposit H3/H4 dimers or tetramers. Throughout the cell cycle, ASF1-HIRA is responsible for the incorporation of H3 and H4, whereas ASF1-CAF1 is involved in replication-dependent histone deposition [114–116]. Upon UV damage induction, ASF1 promotes nucleosome assembly together with CAF1 in a NER-dependent manner [117–119]. CAF1 knockdown does not inhibit NER in mammalian cells, suggesting that this H3.1 deposition is part of a chromatin restoration step after damage has been repaired which likely has no or limited influence on the repair rate itself.
In yeast, ASF1 and CAF1 are also involved in the response to UV irradiation. Both cac1 (the yeast CAF1 gene) and asf1 mutants are sensitive to UV, but a cac1 asf1 double mutant is more sensitive than either single mutant [120, 121]. This suggests that Asf1 can perform its function in the absence of CAF1 and vice versa, albeit at a lower efficiency.
So far, no evidence has been found for the involvement of an H2A/H2B histone chaperone in NER. Possible candidates for this function are NAP1L1 or FACT, responsible for H2A/H2B deposition during replication and transcription [122–124]. In fact, FACT was recently shown to co-purify in complex with H2AX, DNA-PK and PARP1 and to promote the integration and dissociation of H2AX in a reconstituted nucleosomes experiment . Phosphorylation of H2AX by DNA-PK increased the exchange of H2AX, indicating that FACT might function in histone exchange during DNA repair.
Because factors involved in both DNA repair and transcription require access to DNA, it is not surprising that a number of remodelling proteins and histone modifications that are associated with active transcription are shared with NER (for example, SWI/SNF and H3 acetylation) (see Tables 1 and 2). However, in transcriptionally active chromatin, transcription over a damaged template should be prevented while repair factors should still be allowed to bind the damaged DNA. Upon UV damage induction, RNA synthesis is inhibited by several mechanisms to protect the cell against the production of potentially dangerous proteins or RNAs [125–127]. One of these mechanisms involves phosphorylation, ubiquitination and proteolytic breakdown of RNA polymerase II [128, 129]. Physical removal of RNA polymerase II ensures that transcription does not take place at UV-damaged areas. In a more speculative scenario, a similar chromatin-mediated signal transduction pathway as for cell cycle control, through for example covalent histone modifications and histone displacement, can be envisaged to inhibit transcription in a compromised genome. Although this mode of damage-induced transcriptional control after genomic insult was proposed many years ago, evidence of whether this actually occurs is currently lacking.
It is unlikely that histone modifications and remodelling proteins with functions in preventing access to DNA during transcription inhibition have the opposite function during repair. Therefore, it is surprising to note that a number of chromatin-modification factors linked to NER are associated with transcription inhibition rather than activation (H3 dephosphorylation, H4K20me, H2Aub, CAF1). This may be explained by auxiliary functions of remodelling proteins and histone modifications in transcription inhibition at damaged areas rather than a direct role in repair. Alternatively they may play a role in restoration of chromatin status after repair has taken place. Indeed CAF1 activity at NER sites has been suggested to restore chromatin status after repair is finished rather than enhance repair by its remodelling activity . Similarly, UV-induced and ATR-dependent phosphorylation of H2AX and ubiquitination of H2A both require active NER [39, 40, 88] to elicit the histone PTMs, suggestive of a post-repair event.
We have discussed some of the chromatin remodelling activities that take place in association with NER in light of what is known for repair of DSBs. Within this process, remodelled chromatin, especially by ATM-induced phosphorylation and subsequent ubiquitination of H2AX, has been shown to be a major signal in checkpoint activation and amplification [32, 91–93]. UV irradiation also induced phosphorylation of H2AX and ubiquitination of H2A both in an ATR-dependent fashion, most likely resulting in amplification of checkpoint signalling. Surprisingly and in contrast with DSBs, UV lesions require NER activity prior to activation of ATR signalling [40, 45], histone ubiquitination  and finally phosphorylation of key checkpoint proteins CHK1 and p53 . This absence of chromatin-associated damage signalling might in part explain the extreme predisposition for cancer in naturally occurring NER mutants (for example, in NER-deficient xeroderma pigmentosum patients) on top of severely attenuated damage removal in these patients' cells . There are further interesting differences between chromatin remodelling responses upon DSB induction and NER activation. For example, in yeast, serine 122 of H2A becomes dephosphorylated upon UV irradiation, while after induction of other types of damage this residue is phosphorylated . Interestingly, the checkpoint activator protein p53 also shows differential phosphorylation at a different residue in response to UV and to γ-irradiation [131, 132]. Moreover, NER-induced chromatin changes do not occur at microscopically discernable sub-nuclear structures such as IRIF that play an important role in the DDR of DSBs. One of the main challenges in the field of DDR research will be to identify further differences and similarities between responses to different types of lesions and to more precisely determine their functions.
There is an obvious connection of the DDR with chromatin modifications. However, our current knowledge is based on studies which each focus on a separate DDR factor or different aspect of chromatin remodelling. In order to obtain better insight into the complex network of chromatin-associated DDR, a more systematic approach should be employed by combining genetic screening, transcriptional profiling, proteomic analysis and imaging approaches to study the spatio-temporal organisation of the entire DDR. Chromatin immunoprecipitation and mass spectrometry will give us further insight into chromatin components and their modifications in response to genomic insults.
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The authors thank Dr JHJ Hoeijmakers for critical reading and comments, and Dr R Tan and Mr N Wijgers for artwork. CD, ABH and WV were supported by The Dutch Organisation of Scientific Research (NWO); ZonMW grants 912-03-012, 917-46-371 and 917-46-364; and by ESF, EurDyna 855-01-072 and EU FP6 LSHG-CT-2005-512113.
The authors declare that they have no competing interests.
CD wrote the initial draft and ABH and WV edited the manuscript. All authors have read and approved the final manuscript.
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Dinant, C., Houtsmuller, A.B. & Vermeulen, W. Chromatin structure and DNA damage repair. Epigenetics & Chromatin 1, 9 (2008). https://doi.org/10.1186/1756-8935-1-9
- Chromatin Remodelling
- Nucleotide Excision Repair
- Histone Chaperone
- Covalent Histone Modification
- Nucleotide Excision Repair Factor