Fig. 6
From: Deformation of the nucleus by TGFβ1 via the remodeling of nuclear envelope and histone isoforms
Depletion of LMNA reduced clustering of H3K27me3 and histone H1. A Representative confocal microscopy images of LMNA_WT and LMNA_KO Huh7 cells treated with/without TGFβ1 and immunostained using H3K27me3, H1, and lamin B antibodies, and Hoechst 33342. B Cells treated as described in (A) were subjected to PLA analysis. Each fluorescent dot represents the colocalization between H3K27me3 and histone H1. C Representative images showing the number and integrated intensity of the PLA fluorescent dots in a nucleus using MetaMorph® software; (upper) original fluorescence image; (lower) processed image. The average intensity of each dot is color-coded according to the scale on the right. D Number of PLA dots in each cell. E Average integrated intensity of each dot in each cell. Cell number calculations in (D) and (E) were: LMNA_WT, n = 31; LMNA_WT + TGFβ1, N = 38; LMNA_KO, n = 30; LMNA_KO + TGFβ1, N = 40. All images are the sum of z-stacks