Developmental programming of DNA methylation and gene expression patterns is associated with extreme cardiovascular tolerance to anoxia in the common snapping turtle

Ruhr, Ilan; Bierstedt, Jacob; Rhen, Turk; Das, Debojyoti; Singh, Sunil Kumar; Miller, Soleille; Crossley, Dane A.; Galli, Gina L. J.

doi:10.1186/s13072-021-00414-7

Epigenetics & Chromatin

Table 7 Results from a two-way ANCOVA for mRNA expression in ventricles from juvenile snapping turtles

From: Developmental programming of DNA methylation and gene expression patterns is associated with extreme cardiovascular tolerance to anoxia in the common snapping turtle

Gene	Oxygen treatment	Age	Oxygen treatment × age	Covariate
CALR	F_1,20 = 0.27, p = 0.61	F_1,20 = 4.5, p = *0.05*	F_1,20 = 0.18, p = 0.68	F_1,20 = 0.71, p = 0.41
COL8A1	F_1,20 = 6.9, p = *0.02*	F_1,20 = 15.5, p = *0.0008*	F_1,20 = 3.6, p = 0.07	F_1,20 = 8.3, p = *0.009*
DDIT4L	F_1,20 = 9.3, p = *0.006*	F_1,20 = 0.21, p = 0.65	F_1,20 = 0.20, p = 0.66	F_1,20 = 14.4, p = *0.001*
HIF1A	F_1,20 = 3.85, p = 0.06	F_1,20 = 0.04, p = 0.85	F_1,20 = 6.0, p = *0.02*	F_1,20 = 0.96, p = 0.34
HTRA3	F_1,19 = 5.2, p = *0.03*	F_1,19 = 3.9, p = 0.06	F_1,19 = 6.2, p = *0.02*	F_1,19 = 6.0, p = *0.02*
ITGA11	F_1,20 = 5.4, p = *0.03*	F_1,20 = 4.4, p = *0.05*	F_1,20 = 0.66, p = 0.43	F_1,20 = 0.95, p = 0.34
MIPEP	F_1,20 = 8.2, p = *0.01*	F_1,20 = 0.14, p = 0.71	F_1,20 = 0.04, p = 0.85	F_1,20 = 8.9, p = *0.007*
MNAT1	F_1,20 = 9.2, p = *0.006*	F_1,20 = 3.1, p = 0.09	F_1,20 = 0.48, p = 0.5	F_1,20 = 0.08, p = 0.78
NCOA2	F_1,20 = 5.9, p = *0.02*	F_1,20 = 5.1, p = *0.03*	F_1,20 = 2.26, p = 0.15	F_1,20 = 7.9, p = *0.01*
PFKFB1	F_1,20 = 20.8, p = *0.0002*	F_1,20 = 10.4, p = *0.004*	F_1,20 = 15.4, p = *0.0008*	F_1,20 = 2.2, p = 0.15
PPIA	F_1,20 = 5.3, p = *0.03*	F_1,20 = 3.3, p = 0.08	F_1,20 = 0.24, p = 0.63	F_1,20 = 0.17, p = 0.69
SCN5A	F_1,17 = 4.6, p = *0.05*	F_1,17 = 0.04, p = 0.85	F_1,17 = 7.3, p = *0.015*	F_1,17 = 16.5, p = *0.0008*
SNTB1	F_1,20 = 0.05, p = 0.82	F_1,20 = 4.3, p = *0.05*	F_1,20 = 0.79, p = 0.38	F_1,20 = 0.003, p = 0.96
TNNT2	F_1,20 = 6.0, p = *0.02*	F_1,20 = 0.01, p = 0.93	F_1,20 = 0.13, p = 0.72	F_1,20 = 0.12, p = 0.74
TPM3	F_1,20 = 12.5, p = *0.002*	F_1,20 = 1.6, p = 0.22	F_1,20 = 0.32, p = 0.58	F_1,20 = 1.8, p = 0.19
WNT11	F_1,20 = 4.6, p = *0.04*	F_1,20 = 3.4, p = 0.08	F_1,20 = 0.01, p = 0.92	F_1,20 = 1.3, p = 0.27

Oxygen treatment, age, and the oxygen treatment by age interaction were fixed effects in the model. The first principal component from a principal components analysis of CACNA2D1, CNP, and YTHDF3 expression was used as the covariate in the ANCOVA. Significant effects (p ≤ 0.05) are highlighted in bolditalics

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ISSN: 1756-8935

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