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Fig. 7 | Epigenetics & Chromatin

Fig. 7

From: The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila

Fig. 7

Interaction network of Nrp1-HA and direct interaction of Nrp1 and Asf1. A Interaction network view of Nrp1-HA. The bait node is shown in purple. The distance and location of the prey node are shown according to their frequency and putative functions. B The structure of the Nrp1-Asf1 complex was predicted by Z-DOCK. The predicted structures and lipotropic property were viewed and rendered using PyMOL. C 12.5% SDS-PAGE analysis of His-Nrp1 and GST-Asf1. M: protein molecular weight markers; lane 1: purified His-Nrp1; lane 2: purified GST-Asf1; lane 3: purified GST. D Pull down assay confirms the interaction between Nrp1 and Asf1. Recombinant His-Nrp1 and GST-Asf1/GST were incubated with glutathione sepharose resin. Unbound proteins were washed off. The protein complexes were eluted and immunoblotted with anti-GST and anti-His antibodies, respectively

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Epigenetics & Chromatin

ISSN: 1756-8935