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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5

Fig. 2

Xdj1, Ssa1, and Ssa2 are dispensable for silencing of the pericentromeric marker gene. a, b Silencing assay of Hsp40 mutants (a) and of Hsp70 mutants (b). Cells were serially diluted, spotted on normal YES plates (YES) and YES plates containing limited amounts of adenine (Low adenine), and incubated at 30 °C for 3 days. c Strand-specific RT-qPCR for the pericentromeric ade6 marker gene. Values are presented as means + SD (n = 3)

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Epigenetics & Chromatin

ISSN: 1756-8935