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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70

Fig. 2

Asx co-fractionates and co-immunoprecipitates with Trx in embryo nuclear extracts. a Schematic of Bio-Rex-70 fractionation of embryo nuclear extracts and coupled co-immunoprecipitation–western blot analysis. b Western blot analysis of Bio-Rex 70 fractions with anti-Asx (upper panel), anti-Trx (middle panel) and anti-E(z) (lower panel). c Coupled anti-Asx immunoprecipitation and anti-Trx western blot analysis of the BR70-0.85 fraction, showing Asx association with Trx. d Coupled anti-Asx immunoprecipitation and anti-E(z) western blot analysis of the BR70-0.1 fraction, showing lack of Asx association with E(z) in the enriched E(z) fraction. e Coupled anti-Asx immunoprecipitation and anti-E(z) western blot analysis of the BR70-0.85 fraction, showing lack of Asx association with E(z) in the enriched Trx fraction

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Epigenetics & Chromatin

ISSN: 1756-8935