Figure 4

Assays for cytosine modifications and a targeted sequencing strategy. We show regular bisulphite sequencing, which reports both 5mC and 5hmC, and two assays detecting 5hmC alone, as examples of the broader group of assays detecting cytosine variants. Any system that captures bisulphite converted DNA can be used downstream of these sample preparation approaches to target deep sequencing to loci of interest, here indicated by cis-regulatory elements (a). In (b) we show the steps and the nucleotide conversions involved in bisulphite sequencing, TAB-seq and oxBS-seq.