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Table 1 Polymerase (Pol II) phosphorylation dynamics and H3K4me2 erasure in primordial germ cells (PGCs)

From: Trans-generational epigenetic regulation of C. elegans primordial germ cells

Genotype

H5 staining positive, % (n/total n)

H3K4me2 positive, % (n/total n)

Wild type (N2)

7 (4/54)

0 (0/37)

mes-2 (bn11)

9 (3/35)

3 (1/40)

mes-4 (bn67)

71 (32/45)

71 (15/21)

mes-4 (bn85)

65 (15/23)

78 (29/37)

  1. Scoring results of late stage embryos with hyperphosphorylated Pol II (H5 staining) or H3K4me2-positive PGCs. Embryos at ~1.5-f to 2-fold stage were examined within a ~40 minute developmental window at around 500 minutes postfertilization at 20°C. A strict criterion was used to assess antibody signal: only those embryos with PGCs that showed comparable levels of H5 or anti-H3K4me2 staining relative to the staining in surrounding somatic cells were scored as 'positive'.
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Epigenetics & Chromatin

ISSN: 1756-8935