Fig. 3

Constitutive heterochromatin marks and their nuclear distribution in gonadal germ cells. A H3K9me3 in chicken embryo gonads. Immunodetection of H3K9me3 (gray) and germ cell marker (red) in tissue sections. DNA staining (cyan). Scale bar: 10 µm. Quantification of fluorescence intensity in germ and somatic cell nuclei is shown below. Number of analyzed nuclei in 6‑, 8‑, 10‑, and 14‑day-old embryos: for females, 61, 53, 34, and 91 germ cells and 685, 423, 311, and 210 somatic cells, respectively; for males, 88, 46, 64, and 40 germ cells and 377, 266, 183, and 229 somatic cells, respectively. B Immunodetection of H3K9me3 (magenta) and HP1beta and gamma (green) in a 10‑day-old male embryo gonadal tissue section. Stars indicate germ cell nuclei, identified using their specific H3K9me3 enrichment and nuclear morphology. DNA staining (cyan). Scale bar: 5 µm. C Analysis of the radial distributions of H3K9me3, HP1, and DNA signal intensities in germ and somatic cell nuclei. Number of analyzed nuclei for germ and somatic cells: 44 and 55 for H3K9me3, 44 and 55 for DNA, 25 and 30 for HP1alpha, and 19 and 25 for HP1gamma, respectively. D Quantification of fluorescence intensity for HP1beta and gamma immunodetection in gonadal germ and somatic cell nuclei from 10-day-old male embryos. Number of analyzed nuclei for germ and somatic cells: 27 and 60 for HP1beta, and 19 and 45 for HP1gamma, respectively