Table 1 Online HPLC parameters for histones H4 and H3
Histone H4 | Histone H3 | |
|---|---|---|
Sample Volume | 1 µL | 1 µL |
Temperature | 4.0 [°C] | 4.0 [°C] |
Buffer A | 2% ACN 0.1% FA | 2% ACN 0.1% FA |
Buffer B | 98% ACN 0.1% FA | 98% ACN 0.1% FA |
Equilibration | Duration = 5.000 [min] | Duration = 5.000 [min] |
Column | 10 cm × 100 µm self-packed C3 | 10 cm × 100 µm self-packed C3 |
Flow rate | 0.200 [µL/min] | 0.200 [µL/min] |
Linear Gradient | Time (min) | % Buffer B [%] | Time (min) | % Buffer B [%] |
|---|---|---|---|---|
0 | 29.0 | 0 | 4.0 | |
0.5 | 29.0 | 0.5 | 4.0 | |
70 | 35.0 | 70 | 15.0 | |
75 | 98.0 | 105 | 35.0 | |
80 | 98.0 | 110 | 98.0 | |
85 | 5.0 | 115 | 98.0 | |
120 | 5.0 |