Fig. 6

Histone H3.2 K9me2- or K9me3-containing proteoforms increase in brown adipose tissue in response to cold housing. (A) Selected discrete H3.2 PTM abundance at different housing temperatures. (B) Proteoform changes between SC and TN. Dot size corresponds to absolute percentage point change. Welch’s t-test was used for volcano plot data, with cutoffs of |1.5| for fold change and p < 0.05. Red dots represent both p < 0.05 and fold change >|1.5|; blue dots represent p < 0.05 and fold change <|1.5|; grey dots represent p > 0.05 and fold change >|1.5|; black dots represent p > 0.05 and fold change <|1.5|. (C) Ternary (3-PTM) combinations show significant differences with K9 di- and tri-methylation, K23 acetylation, and K36 monomethylation. n = 3–5 per group. One-way ANOVA testing was conducted for all data comparing TN, RT, and SC housing conditions, and p-values are indicated in each plot. pp: percentage point. TN: thermoneutral. RT: room temperature. SC: severe cold