Fig. 5

Under normal room temperature housing conditions, BAT histone H3.2 and H4 post-translational modifications and proteoforms are distinct from those observed in liver. (A) Histone H3.2 and (B) histone H4 binary PTM combinations in BAT and liver. (C) Dot size references for binary volcano plots (A, B). (D) Histone H3.2 and (E) histone H4 proteoforms from BAT and liver. (F) Dot size references for proteoform volcano plots (D,E). P-values were calculated using Welch’s t-test. Volcano plots show cutoffs of |1.5| for fold change and p < 0.05. Red dots represent both p < 0.05 and fold change >|1.5|; blue dots represent p < 0.05 and fold change <|1.5|; grey dots represent p > 0.05 and fold change >|1.5|; black dots represent p > 0.05 and fold change <|1.5|. n = 3–5 per group. RT: room temperature