Fig. 2
Alternative splice variants and reduced representation bisulfite sequencing (RRBS) data reveal changes in brown adipose tissue associated with housing temperature. (A) A comparison of SC vs TN housing RNA-Seq data reveals differential expression of alternate splice variants in BAT. Integrated RNA-Seq and RRBS data for (B) SC vs TN housed mice. (C) SC vs RT housed mice; and (D) RT vs TN housed mice. n = 4 per group for RNA-Seq and RRBS datasets. RRBS data shown are for percent methylation difference >|5|, padj < 0.05. DEG: differentially expressed genes. DMP: differentially methylated promoters. DMG: differentially methylated genes. TN: thermoneutral. RT: room temperature. SC: severe cold