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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Transcriptionally active chromatin loops contain both ‘active’ and ‘inactive’ histone modifications that exhibit exclusivity at the level of nucleosome clusters

Fig. 4

H3K4me3 and H3K27me3 distributions. A)&B) LSCM of H3K4me3 and H3K27me3 labelled spermatocytes. The ImageJ smooth filter was applied. (A) overview, maximum intensity projection of 4 slices (Δ z = 300 nm). Bar, 3 μm. (B) Detail of boxed region in A) showing a fibre emanating from the nucleolus and carrying both histone marks. Single optical section. C)-E): N2V denoised STED images. (C) overview. D)&E) details, 3 optical sections (Δ z = 450 nm). (D) shows fibres in the same nucleus almost exclusively stained either for H3K4me3 or H3K27me3. Images are rotated by 90° relative to C). (E) Detail from another cell showing H3K27me3 clusters along a fibre with locally associated H3K4me3. Scale bars, 1 μm

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Epigenetics & Chromatin

ISSN: 1756-8935