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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Transcriptionally active chromatin loops contain both ‘active’ and ‘inactive’ histone modifications that exhibit exclusivity at the level of nucleosome clusters

Fig. 1

H3K4me3 distribution along Y loops. STED immunofluorescence (IF) microscopy of histones (magenta and central panel) and H3K4me3 (green and right panel). (A) overview of a spermatocyte nucleus, N2V denoised and maximum projected over 5 μm (10 slices with Δz = 0.5 μm). The Y loops can be identified as chromatin fibres in the central nucleoplasm, distinct from the chromosome masses of the X and Y chromosomes close to the nucleolus and the autosome chromosomes which are confined to the nuclear periphery. no, nucleolus with sex chromosomes; au, dense autosome masses at the nuclear periphery. (B) Detail of boxed region in A) showing a single optical unfiltered (raw) section of a Y loop fibre enriched with H3K4me3 and one without H3K4me3 nearby (ellipses). (C) Example of a fibre with one H3K4me3 enriched segment in another cell. Maximum projection of 4 unfiltered (raw) slices. Of note, the same STED laser was used for both excitation lasers, thus eliminating the potential for any artificial shifts between the two fluorophores due to misalignment. Scale bars, 1 μm

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Epigenetics & Chromatin

ISSN: 1756-8935