Fig. 5
From: TP63–TRIM29 axis regulates enhancer methylation and chromosomal instability in prostate cancer
Restoration of TRIM29 expression promotes decrease in genome instability in PRAD. A qRT-PCR analysis of TMPRSS2:ERG fusion transcripts from the LNCaP cells with the indicated treatments for 48 h. TP testosterone propionate. B γH2AX foci formation upon treatment of LNCaP and LNCaP-TRIM29 cells with TNFα (100 ng/mL). Cells were processed 48 h post-treatment. Scale bar indicates 25 μm. C Violin plots of the γH2AX foci number per nucleus in LNCaP and LNCaP-TRIM29 cells before and after TNFα (100 ng/mL) treatment. D Immunoblot (IB) analysis of lysates from LNCaP and LNCaP with stably expressed TRIM29 cells stimulated with TNFα (100 ng/mL)