Fig. 4

TRIM29 interacts with TP63 and regulates the expression of the TP63 cluster. A Eigengene of TP63 cluster significantly correlates with the expression of TRIM29. B TP63 expression significantly correlates with the expression of TRIM29 in four PRAD datasets. C Comparison of TP63 and TRIM29 knockdowns (KDs). GSEA plots evaluating the TP63 cluster signature upon TRIM29 knockdown in RWPE-1 (prostate basal epithelium, left-top) and HMEC (GSE71375, basal breast epithelium, left-bottom). Venn diagram showing the overlap of differentially expressed TP63 cluster genes upon TP63 and TRIM29 knockdown in the RWPE-1 cells (right). NES Normalized enrichment scores. D Comparison of TP63 and TP63 + TRIM29 overexpression (OEs). GSEA plots evaluating the TP63 cluster signature upon TRIM29 overexpression in PC3 (PRAD, left-top) and TP63 and TRIM29 simultaneous overexpression in PC3 (PRAD, left-bottom). Venn diagram showing the overlap of differentially expressed TP63 cluster genes upon TP63 overexpression and TP63 and TRIM29 simultaneous overexpression in PC3 cells (right). E ChIP-seq profiles of H3K27ac (top track) and TP63 (bottom track) in the RWPE-1 cell line of the TRIM29 gene. Enhancer 1 of TRIM29 in a red polygon. F Immunoblot (IB) analysis of knockdown (KD) of TP63 and TRIM29 (left) and TP63 and TRIM29 overexpression (OE, right) in the RWPE-1 cells. G Immunoblot (IB) analysis of overexpression (OE) of TP63 and TRIM29 in PRAD cell lines: LNCaP, 22Rv1, and PC3. H Interaction between TRIM29 and TP63 was detected by co-immunoprecipitation (co-IP) in the PC3 cells overexpressing TRIM29-FLAG and TP63. I Overexpression (OE) of TRIM29 does not lead to the relocation of TP63 from the nucleus to the cytoplasm. Immunocytochemistry staining of TP63 in the RWPE-1 cells overexpressing TRIM29. J Immunoblot (IB) analysis of overexpression (OE) of TP63 and TRIM29 simultaneously in PC3 cells