Fig. 5

Sin3B promotes a permissive environment for differentiation. ATAC-Seq was performed on isolated LT-HSCs purified via FACS. Differentially accessible regions were calculated using the Diffbind package in R. A Heatmap of differentially accessible peaks in Sin3B^F/F LT-HSCs. Differential accessibility refers to statistically significant peaks between genotypes. Shown are 287 peaks identified as more open in Sin3B^F/F LT-HSCs. Each column represents peaks from one replicate (2 mice) of the indicated genotype. B Heatmap of differentially accessible peaks in Sin3B^−/− LT-HSCs. Shown are 5531 peaks identified as more open in Sin3B^−/− LT-HSCs. C Venn diagram showing overlap of non-promoter peaks from dataset, overlapped with a list of published primed peaks from Martin, et al. D Primed peaks unique to wild-type LT-HSCs were analyzed with the GREAT tool and the list of genes that are putatively regulated by these regions were analyzed using Enrichr. Shown are the pathways identified by BioPlanet as significantly enriched. E Homer motif analysis for primed peaks unique to Sin3B^+/+ LT-HSCs. Shown are the top 3 motifs and p-values. F Representative tracks for the gene Ebf1, a gene regulated by one of the primed peaks identified in (D) the cis-regulatory element is highlighted. G Representative tracks for the gene Ntrk3, a gene regulated by one of the primed peaks identified in (D) the cis-regulatory element is highlighted. Each track represents data from one experimental point