Fig. 3

Sin3B^−/− LSKs display defective stress hematopoiesis. A LSKs were sorted from Sin3B^F/F and Sin3B^H−/− mice 9 days after 5-FU administration (100 mg/kg, i.p.) and subjected to scRNA-Seq analysis utilizing the same strategy as in Fig. 1. n = 2 mice per genotype. B Sin3B^+/+ and Sin3B^−/− cells were able to be separated in our data based on hashtag oligonucleotide sequencing. C Quantification of different hematopoietic subsets at indicated conditions. D Monocle3 differentiation trajectory analysis of Sin3B^+/+ and Sin3B^−/− LSKs during a stress response. E Pseudotime analysis utilizing LT-HSCs as starting node for either Sin3B^+/+ and Sin3B^−/− LSKs. F Differential expression analysis was used to calculate modules of genes grouped together based on expression as a function of pseudotime. G Gene Ontology analysis for 100 genes in common. H Gene Ontology analysis for 136 genes unique to Sin3B^+/+ LT-HSCs. i Gene Ontology analysis for 126 genes unique to Sin3B^−/− LT-HSCs