Fig. 5

Native detection of DNA modifications with nanopore long-read sequencing. Nanopore long-read sequencing was performed on INTACT-isolated high molecular weight gDNA from neurons, astrocytes, and microglia (n = 2/group). Native mCG and hmCG calling was performed to obtain total whole genome %mCG A and %hmCG B. %modC (%mCG or %hmCG) was plotted across chromosome 15 C to demonstrate modification differences between cell types and reproducibility across biological replicates. Modification values were smoothed in CpG-only coordinate space (One-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01)