Fig. 4

Comparison of DNA modifications across three CNS cell types. Whole genome total CG modifications A and CH modifications B from INTACT-isolated gDNA from neurons (hippocampus), astrocytes (half brain), and microglia (half brain) were compared to two single nuclei methylome studies [49, 52]. C TRAP RNA-seq expression of DNA modification regulators in neurons, astrocytes, and microglia (n = 3–6/group; one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) data presented at reads per kilobase mapped. D–F Whole genome, repetitive element, and non-repetitive element mCG, hmCG, and mCH levels for neurons, astrocytes, and microglia. (n = 4/group; two-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)