Fig. 3
From: Differential usage of DNA modifications in neurons, astrocytes, and microglia
Profile of hippocampal neuronal DNA modifications by whole genome oxBS-seq. INTACT hippocampal gDNA from input, negative and positive fractions was taken for whole genome bisulfite and oxidative bisulfite sequencing. A–D Total genomic levels of mCG, hmCG, mCH, and hmCH (n = 4/group; one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001). Levels of mCG were lower and hmCG and mCH were higher in the positive fraction. E–G mCG, hmCG, and mCH averaged over 200 nucleotide bins from 4 kb upstream, within the gene body, and 4 kb downstream of neuronal marker genes in the positive fraction and input. H–J Average mCG, hmCG, and mCH for positive fraction and input 4 kb upstream of the TSS, within the gene body, and 4 kb downstream of the TES of neuronal genes revealed lower mCG and higher hmCG and mCH in gene bodies and downstream (n = 4/group; paired two-tailed t-test between input and positive fraction, *p < 0.05, **p < 0.01, ***p < 0.001)