Fig. 2
From: Differential usage of DNA modifications in neurons, astrocytes, and microglia
Validation of neuronal genome enrichment in Camk2a-NuTRAP mouse hippocampus by INTACT-BS seq. A–C Confocal fluorescent microscopy images from positive, input, and negative INTACT nuclei isolations. D INTACT-isolated gDNA from the hippocampus of Camk2a-NuTRAP mice was bisulfite converted and whole genome levels of CG modifications measured for input, negative, and positive fractions. CG modifications from previously published neuronal methylation studies utilizing various brain regions (hippocampus and cortex) and isolation techniques (Camk2a INTACT, NeuN+ sorting, and single cell) were compared to Camk2a-NuTRAP CG modifications. E Whole genome CH modifications were measured for input, negative, and positive fractions. CH modifications from the same neuronal methylation studies from D were compared to Camk2a-NuTRAP CH modifications (**p < 0.01 by one-way ANOVA with Tukey’s multiple testing correction)