Fig. 10

Verification of differentially hydroxymethylated regions. Targeted enzymatic methyl-seq (EM-seq) was performed from INTACT-isolated gDNA from neurons, astrocytes, and microglia (n = 3/group) in six regions found to be differentially hydroxymethylated with oxBS. Line plots and total hmCG were plotted for regions corresponding to Chn1 A, Dlgap1 B, Ankrd33b C, Dab2ip D, Chst2 E, and Kalrn F (two-way ANOVA with Sidak’s multiple testing correction and single pooled variance for individual CpG differences between cell types, two-tailed unpaired t-test for average region differences between cell types; *p < 0.05, **p < 0.01, ***p < 0.001)