Fig. 1

Oocyte Dnmt3l expression and promoter sequence. a Expression of Dnmt3 genes across mammalian oocytes using available RNA-seq data sets. RPKM = reads per kilobase of transcript per million reads in the library (in paired-end libraries, fragments instead of reads were quantified). Dots represent individual data points (biological replicates), error bars show standard deviation. b Ensembl genome browser screenshot of the mouse Dnmt3l gene and its promoter region in single bp resolution. c Screenshot of reverse complement of Aire intron 3 multiple sequence alignment (only the sequence alignment around TSS and presumable TBPL2/TFIIA binding site is shown) in mammals with available oocyte RNA-seq data sets. The Dnmt3l oocyte TSS is highlighted in green, the upstream CT-rich region and downstream GA-rich region surrounding the TSS are highlighted in grey and the presumable TBPL2/TFIIA binding site is highlighted in yellow. Ro rodents, Pr primates, Un ungulates. d Visualisation of the TBPL2/TFIIA consensus binding site (adapted from [66] and matching sequences identified in mouse oocyte Dnmt3l TSS. Top sequence (I.)) represents the sequence with exactly matching positions upstream of TSS, bottom sequence (II.)) represents best fit for the binding site positioned 3 bp upstream of the exact match. The numbers indicate the position in the mouse sequence respective to the Dnmt3l TSS. e Screenshot from the program Seqmonk showing SLIC–CAGE read quantification of the first 200 bp from mouse Dnmt3l TSSs in control and TBPL2 knock-out oocytes [66]. RPM reads per million reads in the library