Fig. 6

MTX reduced levels of Dot1l and H3K79me2 in NTDs mice. A Low folate feed and MTX injection induced NTDs in C57BL/6 mouse embryos at 13.5 days. B The brain and spinal tissues of mouse embryos induced by MTX were obtained, collect proteins from the tissues were extracted, Dot1l and H3K79me2 detection by western blot. C RT-qPCR was used to detect the mRNA levels of Dot1l in brain and spinal tissues of NTDs mice. Data are mean ± s.d. (n = 3). *p < 0.05. D Representative images from immunohistochemical staining of H3K79me2 in the cranial neural tissue from E10.5. E H3K79me2 expression scores are shown as box plots, with the horizontal lines representing the median. Data are mean ± s.d. (n = 3), *p < 0.05, by Student’s t test