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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Folate deficiency reduced aberrant level of DOT1L-mediated histone H3K79 methylation causes disruptive SHH gene expression involved in neural tube defects

Fig. 5

Folinic acid attenuates MTX-reduced enrichment of H3K79me2 in Shh, Sufu genes. A Western blot assay analyzed as indicated antibody. mESCs were cultured in complete medium under MTX treatment (1 μM; 12 h), and supplemented with folinic acid (50 mg/L; 24 h). B Immunofluorescence was performed on indicated antibodies H3K79me2 and Dot1L in mESCs under MTX (1 μM; 12 h) treatment and folinic acid supplementation. C mESCs cells were treated with MTX and replenishing with folic acid, and the mRNA levels of Shh-related genes Shh, Sufu was detected in RT-qPCR. All data were obtained in three repeated experiments (n = 3). *p < 0.05. D mESCs were treated with MTX and supplemented with folic acid, ChIP experiments of H3K79me2 were performed, and the enrichment of H3K79me2 in Shh, Sufu genes were observed by ChIP-qPCR. All data were obtained in three repeated experiments (n = 3), *p < 0.05

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Epigenetics & Chromatin

ISSN: 1756-8935