Fig. 1

Snhg15 is associated with DNMT1 in an ephrinA5-dependent manner in CB cells. a Location of the three Snhg15 amplicons used to amplify CLIP-RNA (from left to right: SP2, SP3, SP1) and the DNA-binding domain (DBD) location. b RNA recovery for IgG and anti-DNMT1 antibody CLIP samples in CB cells (N = 5 biological replicates). For amplicon positions SP1, SP2, and SP3, CLIP clearly indicated a (functional) association of DNMT1 with Snhg15, and a reduction of the amount of bound Snhg15 RNA after ephrinA5-Fc stimulation. Whiskers of the box plots extend to the minimum and the maximum of the data values from the 25th and 75th percentiles (Spear style). Significances were determined with two-tailed Student’s t-test. Significance levels: p value < 0.05 *; p value < 0.01 **; p value < 0.001 ***. ctrl-Fc: control Fc, efnA5-Fc: ephrinA5-Fc, CLIP: UV cross-linking and immunoprecipitation