Development of super-specific epigenome editing by targeted allele-specific DNA methylation

Table 1 Compilation of ASM experiments conducted in this study

No.	Gene	SNP location	Experiment	SNP	Target allele	ASM (Δ%)	ASM ratio
1	DAP3	PAM3	DAP3-PAM3	T to G	G	44	4.4
2	DAP3	Seed	DAP3-Seed1	T to G	G	15	1.4
3	DAP3	Seed	DAP3-Seed2	T to G	T	27	5.6
4	GPD1L	PAM3	GPD1L-PAM3	G to T	G	50	7.6
5	GSPT1	PAM3	GSPT1-PAM3	T to G	G	47	7.1
6	GSPT1	Seed	GSPT1-Seed1	T to G	G	18	1.7
7	GSPT1	Seed	GSPT1-Seed2	T to G	T	28	2.1
8	ISG15	PAM2	ISG15-PAM2	C to G	G	63	8.5
9	ISG15	Seed	ISG15-Seed1	C to G	G	36	2.3
10	ISG15	Seed	ISG15-Seed2	C to G	C	34	1.8
11	MAPK1	Seed	MAPK1-Seed1	T to G	T	19	1.4
12	MRPL52	PAM3	MRPL52-PAM3	G to T	G	52	3.7
13	MSH6	PAM3	MSH6-PAM3	T to G	G	46	10
14	MYH10	Seed	MYH10-Seed1	G to A	A	16	2.6
15	NARF	PAM2	NARF-PAM2	G to T	G	45	5.6
16	NARF	Seed	NARF-Seed1	G to T	G	43	3.0
17	NARF	Seed	NARF-Seed 2	G to T	T	31	1.7
18	PDE8A	PAM2	PDE8A-PAM2	G to T	G	19	3.1
19	PDE8A	Seed	PDE8A-Seed1	G to T	G	26	1.9
20	PDE8A	Seed	PDE8A-Seed2	G to T	T	45	4.4
21	RAF1	PAM3	RAF1-PAM3	G to A	G	4	1.7
22	RALB	PAM2	RALB-PAM2	G to C	G	8	2.6
23	TTC41P	PAM3	TTC41P-PAM3	G to T	G	6	2.3
24	TYK2	PAM2	TYK2-PAM2	G to C	G	15	2.7

“ASM (Δ%)” and “ASM (ratio)” refer to the difference and ratio of the increases in DNA methylation at the on- and off-target alleles. The detailed targeting strategy in each case including genome coordinates is shown in Additional file 1

ISSN: 1756-8935