Fig. 6
From: Development of super-specific epigenome editing by targeted allele-specific DNA methylation
Stability of ASM. A Exemplary DNA methylation profiles of GPD1L-PAM3 on-target and off-target alleles on the days 3, 5, 8 and 11 after transfection. The bar graph indicates the average DNA methylation levels at the selected CpG sites. Error bars show the SD of three biological repeats. “scr-sg” refers to samples treated with scrambled sgRNA, “gene-sg” refers to samples treated with the allele-specific sgRNA. B Bar graphs indicating the average DNA methylation levels at the selected CpG sites of ISG15-Seed2, MSH6-PAM3, ISG15-PAM2, MHY10-Seed1, MRPL52-PAM3 and PDE8A-PAM2 on day 3, 5, 8 and 11 after transfection. Error bars show the SD of three independent biological repeats except for except for ISG15-Seed2, MSH6-PAM3 and MYH10 Seed1 which were conducted twice. Corresponding DNA methylation profiles are shown in Additional file 2: Fig. S6