Fig. 4
From: Development of super-specific epigenome editing by targeted allele-specific DNA methylation
Examples of DNA methylation profiles and methylation levels observed after targeting a SNP in the third position of the PAM site. The graphs show the DNA methylation at each CpG site in the different target regions. The CpG sites in the grey regions were included for the calculation of the respective summary bar graphs. “scr-sg” refers to samples treated with scrambled sgRNA, “gene-sg” to samples treated with the allele-specific sgRNA. The sgRNA binding site is indicated by a dark red line. The bar graphs show corresponding levels of average DNA methylation at the selected CpG sites. Error bars show the SD of three independent biological repeats except for MSH6-PAM3 where two repeats were conducted