Fig. 1
From: Development of super-specific epigenome editing by targeted allele-specific DNA methylation
Scheme illustrating the selective binding of the EpiEditor complex to the target allele. A Sequence discrimination by a SNP in the sgRNA seed region. The sequence of allele 1 matches the sgRNA leading to the DNA methylation delivery in the targeted allele. Allele 2 has a nucleotide mismatch in the seed region of the sgRNA, which disfavors the complex assembly. B Sequence discrimination by a SNP in the PAM site. The sequence of allele 1 matches the sgRNA and it has a PAM at the distal end. Hence, the EpiEditor complex assembles at allele 1 leading to the DNA methylation deposition. Although the sgRNA matches the sequence of allele 2, the SNP disrupts the PAM and the absence of the PAM prevents binding of the sgRNA/dCas9 complex