Fig. 5

No evidence of increased L1 retrotransposition upon vitC treatment. A Schematic showing the design of the retrotransposition experiment undertaken. Four clonal cell populations were grown in 2i media or 2i + vitC for 13 or 41 days, followed by L1 targeted sequencing analysis and processing by TEBreak to identify de novo retrotransposition events. B Agarose gel electrophoresis detection of candidate TE insertions following PCR on samples from the 13-day experiment. All 6 analysed insertions were detected in both control and vitC samples of the expected clone, including a candidate de novo insertion. C As in B, but for the 41-day experiment. One bona fide de novo insertion was identified that could be validated by PCR, which occurred in the non-vitC control condition. NTC: no template control. Insertion coordinates are for the mm10 genome build