Fig. 4

L1 activation by vitC is driven by a reduction in H3K9me2/3. A qRT-PCR data (relative to untreated WT cells) showing expression of L1s (and Dazl) in Kdm4a/b/c TKO cells, with and without vitC. P-values refer to the effect of the ‘sample’ variable from an ANOVA model using ‘sample’ and ‘amplicon/target’ as independent variables. B Western blots showing that L1 ORF1p protein upregulation with vitC is lost in Kdm4 TKO cells. C Deep amplicon sequencing from oxBS-treated DNA was used to measure 5mC and 5hmC levels at young L1s in ctrl and vitC-treated Kdm4 TKO mESCs; each data point represents the average value from three biological replicates at a given CpG within the amplicon. P-values refer to the effect of the ‘sample’ variable from an ANOVA model using ‘sample’ and ‘CpG position’ as independent variables. D qRT-PCR data for L1s in shSetdb1 KD mESC, compared to untreated shScr controls, with and without vitC. P-values refer to the effect of the ‘sample’ variable from an ANOVA model using ‘sample’ and ‘amplicon/target’ as independent variables