Fig. 2

TET activity is dispensable for vitC-mediated L1 upregulation. A Deep amplicon sequencing of oxBS-treated DNA was used to measure 5mC and 5hmC levels at young L1s in control and vitC-treated mESCs; each data point represents the average value from three biological replicates at a given CpG within the amplicon. P-values refer to the effect of the ‘sample’ variable from an ANOVA model using ‘sample’ and ‘CpG position’ as independent variables. B qRT-PCR data showing the relative change in expression level (compared to untreated WT cells) for L1s in Tet triple knockout (TKO) cells in control and vitC culture conditions. C qRT-PCR data as in B, but for ERVs. P-values for B and C refer to the effect of the ‘sample’ variable from an ANOVA model using ‘sample’ and ‘amplicon/target’ as independent variables