Fig. 7

Physical interactions of the pol30 mutant proteins with Cac1p and Rrm3p. A Yeast two-hybrid interaction measured as units of β-galactosidase produced per milligram of protein (U/mg of protein). Average values and standard deviations from three independent experiments are plotted on Y-axis as a factor of wild-type protein interactions. The bait and prey plasmids are indicated on the graph. B Co-immunoprecipitation of Pol30p and mutant pol30p by Cac1p-FLAG. Western blot was performed using α-PCNA and α-FLAG antibodies. C Co-immunoprecipitation of Pol30p and mutant pol30p by Rrm3p-Myc. Western blot was performed using α-PCNA and α-Myc antibodies. D Pixels of western blot band images were quantified, and the Pol30p bands were normalized against corresponding Cac1p/Rrm3p band intensities. Average values and standard deviations from two independent experiments with two biological replicates were plotted. Asterisks represent statistical significance compared to the wild-type Pol30p in each IP. *p value 0.05, **p value < 0.001