Fig. 4

Analysis of URA3 gene expression in mutant pol30 strains harboring deletions of CAC1 and ASF1. The analyses were performed using adh4-URA3-yEGFP←HTB1-tel fragment at 0.5 × and 1 × concentrations of 5-FOA as described in the text. The pol30 mutants are listed on the horizontal axis. The graphs in A, C represent average values and standard deviations of three experiments at the indicated concentrations. Asterisks represent statistical significance compared to the wild type within each gene deletion background. *p value 0.05, **p value < .001. B, D The ratio between % FOA^R cells in the POL30 versus the pol30 mutant strains were calculated and plotted from the values in A and C