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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Analyses of POL30 (PCNA) reveal positional effects in transient repression or bi-modal active/silent state at the sub-telomeres of S. cerevisiae

Fig. 3

Fluorescent microscopy analysis of cells harboring the adh4-URA3-HTB1→yEGFP-tel (A) and adh4-URA3-yEGFP←HTB1-tel (B) reporters. A, B Images of indicated strains captured at ×40 resolution. While the signal ratios in the two panels were kept constant, the brightness/contrast of the GFP and MERGE images in the righthand and lefthand panels were altered to better represent the on/off state of yEGFP expression and the differences in intensity of the yEGFP signal between the two constructs. C GFP intensity distribution of strains harboring adh4-URA3-HTB1→yEGFP-tel fragment compared against background intensity. D GFP intensity distribution of strains harboring adh4-URA3-yEGFP←HTB1-tel fragment compared against background intensity. E Percentage of GFP-negative cells from three independent experiments are plotted. Asterisks represent statistical significance compared to the wild-type harboring each fragment. *p value 0.05, **p value < 0.001

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Epigenetics & Chromatin

ISSN: 1756-8935