Fig. 1

Proportions of cells with silenced URA3 (% FOA^R) in strains harboring adh4-URA3-tel (A), adh4-URA3-HTB1→yEGFP-tel (B) and adh4-URA3-yEGFP←HTB1-tel reporters. The analyses were performed with 0.5 × and 1 × concentrations of 5-FOA as described in the text. The pol30 mutants are listed on the horizontal axis. The calculations in A–C represent average values and standard deviations s of three experiments. Asterisks represent statistical significance compared to the wild type within each concentration. *p value 0.05, **p value < 0.001. In D and E the ratio between % FOA^R cells in the POL30 versus pol30 mutant strains was calculated and plotted from the values in A–C. The logarithmic scale of the vertical axis in D and E was chosen to better capture the range of differences at the two concentrations of 5-FOA