Table 1 Published H3.3 single knockouts and their phenotypes
From: Knockout tales: the versatile roles of histone H3.3 in development and disease
Gene knockout | Knockout phenotype | References |
|---|---|---|
H3f3a, gene trap | 50% neonatal mortality, neuromuscular deficits, reduced fertility in males and females | Couldrey et al. [26] |
H3f3a, Cre-LoxP | Reduced size, male infertility due to reduced sperm motility and head/tail defects | Tang et al. [23] |
H3f3a, knockout-first | Almost full embryonic lethality, smaller heads, underdeveloped lungs, some growth defects in heterozygotes | Bush et al. [27] |
H3f3b, Cre-LoxP (Zp3-Cre) | Partial embryonic lethality, abnormal embryonic development, infertility | Yuen et al. [21] |
H3f3b, Cre-LoxP (Hprt1-Cre) | Full embryonic/perinatal mortality, growth defects and male infertility in heterozygotes | Tang et al. [23] |