Fig. 4

H3K79me2 upregulation correlates with changes in the expression of chromatin binding genes upon NPM1 KD. A Individual data points plot showing the fold change (NPM1 KD/CTR) expression of the indicated genes calculated after RT-qPCR upon 3 days of NPM1 KD in N2a cells. Gapdh was used as reference gene for normalization. Statistical analysis was performed on n = 5 biological replicates using an unpaired two tailed and one tailed (Npm1) t-test; *p < 0.05, **p < 0.01. Outliers were identified using a Grubbs method. Error bars represent S.D. B Representative immunoblot analysis of the levels of EZH2 and NPM1 upon NPM1 depletion (NPM1 KD, 3 days) in N2a cells. Ponceau staining was used for total protein normalization. C Quantification of the signal intensity of EZH2 shown in B was done using Fiji (ImageJ; bottom). EZH2 levels were first normalized to the corresponding Ponceau signal and then presented as a ratio over the control values. Statistical analysis was performed on n = 3 biological samples using an unpaired two tailed t-test. *p < 0.05, **p < 0.01. Quantification of NPM1 KD shown in Additional file 1: Fig. S4E. Error bars represent S.D