Fig. 3

H3K79me2 is enriched at chromatin binding genes upon loss of NPM1. A Pie charts depicting the genomic distribution of H3K79me2 peaks in control (CTR, left), and after 3 days of NPM1 KD (right) conditions. B Pie chart depicting the distribution of genomic regions with differential H3K79me2 enrichment upon 3 days of NPM1 KD compared to control (CTR) condition (NPM1 KD/CTR). C Heatmap of regions with increased (left) and decreased (right) H3K79me2 enrichment clustered according to the control, NPM1KD, and NPM1KD/control conditions. Data are normalized by sequencing depth and input control as log2(ChIP/Input). The difference between NPM1KD and control conditions was calculated from RPKM normalized bigwig files as log2(NPM1KD/Control). The metaprofiles (top) show the average log2FC (LFC) of each cluster. D Dotplot of GO-enrichment analysis of differentially H3K79me2-enriched regions. Gene counts and adjusted p-values are indicated at the right corner and the x-axis depicts gene ratios. Threshold for enrichment analysis was adjusted to p < 0.01 and gene ontology of molecular functions were considered for annotation. E Individual data points plot showing the fold change enrichment over control of H3K79me2 calculated after ChIP-qPCR upon 3 days of NPM1 KD (KD/CTR). C3T2.1 is used as negative control region. Statistical analysis was performed on n = 5 biological replicates using an unpaired two tailed t-test. *p < 0.05, **p < 0.01. Error bars represent S.D