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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Nucleophosmin 1 cooperates with the methyltransferase DOT1L to preserve peri-nucleolar heterochromatin organization by regulating H3K27me3 levels and DNA repeats expression

Fig. 1

DOT1L interacts with monomeric NPM1 on chromatin. A DOT1L (DOT1L-FLAG-HA) was overexpressed in N2a cells and immunoprecipitated using HA antibody (left panel) or NPM1 antibody (right panel). IgG antibody was used as control. For DOT1L immune-detection FLAG antibody was used. B Proximity ligation assay (in situ PLA) to visualize in vivo NPM1/DOT1L interaction (red signal) when both proteins are overexpressed in N2a cells. LMNB1 (LAMINB1) and FBL (FIBRILLARIN) were used as controls to mark the nuclear membrane and the nucleoli respectively. DOT1L/NPM1 interaction occurred inside the nucleus (LMNB1) and outside the nucleolus (FBL). (Scale bar 10 µm). C Immunoblot analysis of DOT1L/NPM1 co-IP as in A) from nucleoplasm or chromatin of N2a cell fractions. Total histone 3 (H3) was used to control the purity of the cell fractionation. For DOT1L immune-detection FLAG antibody was used. D Immunoblot analysis of DOT1L/NPM1 interaction in N2a cell extract as in A) in the presence of DNase or RNase, respectively

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Epigenetics & Chromatin

ISSN: 1756-8935