Fig. 2

H2BK12 as the main site of elevated histone crotonylation. A Distribution of proteins corresponding to different crotonylation modification sites in GO secondary classification. B Bubble map of enrichment and distribution of proteins corresponding to different crotonylation modification sites in GO functional classification. C Crotonylation levels of H2BK11, H2BK12, H2BK20 and H2BK34 in CRC patients and healthy controls (n = 40) measured by western blotting using antibodies against the indicated histone crotonylations. Three representative blots were presented. D Quantification of relative histone H2B levels in the 40 pairs of samples tested. The intensity of crotonylation was quantified using the ImageJ software, followed by statistical analysis. Each scale bar represents mean ± SD for 40 samples. Mean data are normalized to H3 (*P < 0.05 vs. healthy controls)